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dc.creatorLomonte, Bruno
dc.creatorMora Obando, Diana
dc.creatorFernández Ulate, Julián
dc.creatorSanz, Libia
dc.creatorPla Ferrer, Davinia
dc.creatorGutiérrez, José María
dc.creatorCalvete Chornet, Juan José
dc.date.accessioned2014-12-15T16:48:08Z
dc.date.available2014-12-15T16:48:08Z
dc.date.issued2015-01
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0041010114006060es_ES
dc.identifier.issn0041-0101
dc.identifier.otheressn:1879-3150
dc.identifier.urihttp://hdl.handle.net/10669/11223
dc.descriptionartículo -- Universidad de Costa Rica, Instituto de Investigaciones Clodomiro Picado, 2015. Investigación apoyada por ICGEB-Italia, CYTED, Ministerio de Economía y Competitividad de España. Este documento es privado debido a limitaciones de derechos de autor.es_ES
dc.description.abstractBothriechis nigroviridis is an arboreal Neotropical pitviper found in Costa Rica and Panam a. A previous proteomic profiling of its venom revealed the presence of proteins with homology to the A and B subunits of crotoxin/Mojave toxin, a heterodimeric phospholipase A2 (PLA2) complex only described in rattlesnake venoms (genera Crotalus and Sistrurus). The native crotoxin-like heterodimer, named nigroviriditoxin, and its A and B subunits were isolated in the present work, and the complete amino acid sequence of the B subunit was determined. The purified A and B components were demonstrated to form a complex when reconstituted under native conditions. Nigroviriditoxin presents features similar to crotoxin, albeit displaying lower toxicity: the A component decreases the PLA2 activity of the B component, and increases its lethal potency in mice. Also in similarity to crotoxin B, nigroviriditoxin B induces myonecrosis. Its 122 amino acid sequence presents 81% identity with crotoxin B. Accordingly, nigroviriditoxin B was cross-recognized by equine antibodies from a Crotalus durissus terrificus antivenom. Phylogenetic analysis shows that the novel PLA2 from B. nigroviridis venom is basal to the branch including all the homologous PLA2 enzymes described in rattlesnakes, and more distant from PLA2s from Bothriechis species. Nigroviriditoxin is the first heterodimeric PLA2 complex found in a non-rattlesnake, Neotropical viperid venom, which displays structural, functional, and immunochemical similarities to crotoxin. The present findings are compatible with the existence of the particular structural trait of crotoxin-like molecules in New World pitvipers before the split of the Meso-South American and the Nearctic clades.es_ES
dc.description.sponsorshipUniversidad de Costa Rica. ICGEB-Italia. CYTED. Ministerio de Economía y Competitividad de España.es_ES
dc.language.isoen_USes_ES
dc.publisherToxicon vol 93:144–154es_ES
dc.subjectSnake venomes_ES
dc.subjectVeneno de serpientees_ES
dc.subjectSustancia peligrosaes_ES
dc.titleFirst crotoxin-like phospholipase A2 complex from a New World non-rattlesnake species: Nigroviriditoxin, from the arboreal Neotropical snake Bothriechis nigroviridises_ES
dc.typeartículo científicoes_ES
dc.identifier.doidoi: 10.1016/j.toxicon.2014.11.235
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-B4-100


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