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Cytotoxicity induced in myotubes by a Lys49 phospholipase A2 homologue from the venom of the snake Bothrops asper: Evidence of rapid plasma membrane damage and a dual role for extracellular calcium

Villalobos Sole, Juan Carlos; Mora Rodríguez, Rodrigo Antonio; Lomonte, Bruno; Gutiérrez, José María; Angulo Ugalde, Yamileth (2007-12)

Acute muscle tissue damage, myonecrosis, is a typical consequence of envenomations by snakes of the family Viperidae. Catalytically-inactive Lys49 phospholipase A2 homologues are abundant myotoxic components in viperid ...

Snake Venomics of the Lesser Antillean Pit Vipers Bothrops caribbaeus and Bothrops lanceolatus: Correlation with Toxicological Activities and Immunoreactivity of a Heterologous Antivenom

Gutiérrez, José María; Sanz, Libia; Escolano, José; Fernández Ulate, Julián; Lomonte, Bruno; Angulo Ugalde, Yamileth; Rucavado Romero, Alexandra; Warrell, David A.; Calvete Chornet, Juan José (2008-10)

The venom proteomes of the snakes Bothrops caribbaeus and Bothrops lanceolatus, endemic to the Lesser Antillean islands of Saint Lucia and Martinique, respectively, were characterized by reversephase HPLC fractionation, ...

Snake Venomics of the Central American Rattlesnake Crotalus simus and the South American Crotalus durissus Complex Points to Neurotoxicity as an Adaptive Paedomorphic Trend along Crotalus Dispersal in South America

Calvete Chornet, Juan José; Sanz, Libia; Cid, Pedro; De La Torre, Pilar; Flores Díaz, Marietta; Dos Santos, María Cristina; Borges, Adolfo; Bremo, Adolfo; Angulo Ugalde, Yamileth; Lomonte, Bruno; Alape Girón, Alberto; Gutiérrez, José María (2010)

We report a comparative venomic and antivenomic characterization of the venoms of newborn and adult specimens of the Central American rattlesnake, Crotalus simus, and of the subspecies cumanensis, durissus, ruruima, and ...

Tissue pathology induced by snake venoms: How to understand a complex pattern of alterations from a systems biology perspective?

Gutiérrez, José María; Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Lomonte, Bruno; Angulo Ugalde, Yamileth; Fox, Jay W. (2010-01)

The study of snake venom-induced local tissue pathology has been predominantly based on experimental designs and concepts that view tissue alterations from a dichotomous, homogeneous and reductionist perspective. Experimental ...

Membrane cholesterol modulates the cytolytic mechanism of myotoxin II, a Lys49 phospholipase A2 homologue from the venom of Bothrops asper

Rangel Hasbún, José Andrés; Quesada, Orestes; Gutiérrez, José María; Angulo Ugalde, Yamileth; Lomonte, Bruno (2011-04-19)

Lys49 phospholipase A2 (PLA2) homologues present in crotalid snake venoms lack enzymatic activity, yet they induce skeletal muscle necrosis by a membrane permeabilizing mechanism whose details are only partially understood. ...

Isolation and characterization of a myotoxic phospholipase A2 from the venom of the arboreal snake Bothriechis (Bothrops) schlegelii from Costa Rica

Angulo Ugalde, Yamileth; Chaves Olarte, Esteban; Rucavado Romero, Alexandra; Gutiérrez, José María; Lomonte, Bruno; Alape Girón, Alberto (1997-03)

A new myotoxic phospholipase A2was isolated from the venom of the arboreal snakeBothriechis schlegelii(formerlyBothrops schlegelii) from Costa Rica, by ion-exchange chromatography on CM-Sephadex.B. schlegeliimyotoxin I is ...

Antivenoms for Snakebite Envenomings

Gutiérrez, José María; León Montero, Guillermo; Lomonte, Bruno; Angulo Ugalde, Yamileth (2011-10)

Animal-derived antivenoms constitute the mainstay in the therapy of snakebite envenoming. Antivenoms are manufactured by immunizing animals, usually horses, with venoms from a single or several medically-relevant snake ...

Comparative study of the cytolytic activity of snake venoms from African spitting cobras (Naja spp., Elapidae) and its neutralization by a polyspecific antivenom

Méndez Madriz, Ileana Sofía; Gutiérrez, José María; Angulo Ugalde, Yamileth; Calvete Chornet, Juan José; Lomonte, Bruno (2011-11)

Venoms of several Naja species found in Sub-Saharan Africa, and commonly known as "spitting cobras", induce a predominantly cytotoxic pattern of envenomings that may evolve into tissue necrosis and gangrene. Cytotoxic ...

Role of enzymatic activity inmuscle damage and cytotoxicity induced by Bothrops asper Asp49 phospholipase A2 myotoxins: are there additional effector mechanisms involved?

Mora Obando, Diana; Díaz Oreiro, Cecilia; Angulo Ugalde, Yamileth; Gutiérrez, José María; Lomonte, Bruno (Peer Journal 2:e569; DOI 10.7717/peerj.569, 2014-09-16)

Viperid venoms often contain mixtures of Asp49 and Lys49 PLA2 myotoxin isoforms, relevant to development of myonecrosis. Given their difference in catalytic activity, mechanistic studies on each type require highly ...

Venom, venomics, antivenomics

Calvete Chornet, Juan José; Sanz, Libia; Angulo Ugalde, Yamileth; Lomonte, Bruno; Gutiérrez, José María (2009)

Venoms comprise mixtures of peptides and proteins tailored by Natural Selection to act on vital systems of the prey or victim. Here we review our proteomic protocols for uncoiling the composition, immunological profile, ...