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Preclinical assessment of a polyspecific antivenom against the venoms of Cerrophidion sasai, Porthidium nasutum and Porthidium ophryomegas: Insights from combined antivenomics and neutralization assays

Gutiérrez, José María; Tsai, Wan-Chih; Pla Ferrer, Davinia; Solano Trejos, María Gabriela; Lomonte, Bruno; Sanz, Libia; Angulo Ugalde, Yamileth; Calvete Chornet, Juan José (2013-03-15)

A polyspecific antivenom is used in Central America for the treatment of envenomings by viperid snakes. This antivenom is generated in horses hyperimmunized with a mixture of venoms from Bothrops asper, Crotalus simus and ...

Myotoxic and cytolytic activities of dimeric Lys49 phospholipase A2 homologues are reduced, but not abolished, by a pH-induced dissociation

Angulo Ugalde, Yamileth; Gutiérrez, José María; Soares, Andreimar Martins; Cho, Wonhwa; Lomonte, Bruno (2005-09-01)

Lys49 phospholipase A2 (PLA2) homologues are myotoxic proteins devoid of catalytic activity. Their toxic determinants map to the C-terminal region 115–129, which plays an effector role in membrane damage. The dimeric state ...

Snake population venomics and antivenomics of Bothrops atrox: Paedomorphism along its transamazonian dispersal and implications of geographic venom variability on snakebite management

Calvete Chornet, Juan José; Sanz, Libia; Pérez, Alicia; Borges, Adolfo; Vargas, Alba M.; Lomonte, Bruno; Angulo Ugalde, Yamileth; Gutiérrez, José María; Chalkidis, Hipócrates M.; Mourão, Rosa H.V.; Furtado, María de Fátima; Moura Da Silva, Ana M. (2011-04-11)

We describe two geographically differentiated venom phenotypes across the wide distribution range of Bothrops atrox, from the Colombian Magdalena Medio Valley through Puerto Ayacucho and El Paují, in the Venezuelan States ...

Clinical and laboratory alterations in horses during immunization with snake venoms for the production of polyvalent (Crotalinae) antivenom

Angulo Ugalde, Yamileth; Estrada Umaña, Ricardo; Gutiérrez, José María (1997-01)

Six horses were immunized with the venoms of Bothrops asper, Crotalus durissus durissus and Lachesis muta stenophrys for the production of polyvalent (Crotalinae) antivenom. During the immunization, clinical and laboratory ...

Venomous snakes of Costa Rica: biological and medical implications of their venom proteomic profiles analyzed through the strategy of snake venomics

Lomonte, Bruno; Fernández Ulate, Julián; Sanz, Libia; Angulo Ugalde, Yamileth; Sasa Marín, Mahmood; Gutiérrez, José María; Calvete Chornet, Juan José (Journal of Proteomics XX (2014) XXX – XXX, Available online 24 February 2014, 2014-02-24)

In spite of its small territory of ~50,000 km2, Costa Rica harbors a remarkably rich biodiversity. Its herpetofauna includes 138 species of snakes, of which sixteen pit vipers (family Viperidae, subfamily Crotalinae), five ...

Snake Venomics of the Central American Rattlesnake Crotalus simus and the South American Crotalus durissus Complex Points to Neurotoxicity as an Adaptive Paedomorphic Trend along Crotalus Dispersal in South America

Calvete Chornet, Juan José; Sanz, Libia; Cid, Pedro; De La Torre, Pilar; Flores Díaz, Marietta; Dos Santos, María Cristina; Borges, Adolfo; Bremo, Adolfo; Angulo Ugalde, Yamileth; Lomonte, Bruno; Alape Girón, Alberto; Gutiérrez, José María (2010)

We report a comparative venomic and antivenomic characterization of the venoms of newborn and adult specimens of the Central American rattlesnake, Crotalus simus, and of the subspecies cumanensis, durissus, ruruima, and ...

Tissue pathology induced by snake venoms: How to understand a complex pattern of alterations from a systems biology perspective?

Gutiérrez, José María; Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Lomonte, Bruno; Angulo Ugalde, Yamileth; Fox, Jay W. (2010-01)

The study of snake venom-induced local tissue pathology has been predominantly based on experimental designs and concepts that view tissue alterations from a dichotomous, homogeneous and reductionist perspective. Experimental ...

Trends in Snakebite Envenomation Therapy: Scientific, Technological and Public Health Considerations

Gutiérrez, José María; Lomonte, Bruno; León Montero, Guillermo; Rucavado Romero, Alexandra; Chaves Mora, Fernando; Angulo Ugalde, Yamileth (2007)

The therapy of snakebite envenomation has been based on the parenteral administration of animal-derived antivenoms. Despite the success of this treatment at reducing the impact of snakebite mortality and morbidity, mostly ...

Cytotoxicity induced in myotubes by a Lys49 phospholipase A2 homologue from the venom of the snake Bothrops asper: Evidence of rapid plasma membrane damage and a dual role for extracellular calcium

Villalobos Sole, Juan Carlos; Mora Rodríguez, Rodrigo Antonio; Lomonte, Bruno; Gutiérrez, José María; Angulo Ugalde, Yamileth (2007-12)

Acute muscle tissue damage, myonecrosis, is a typical consequence of envenomations by snakes of the family Viperidae. Catalytically-inactive Lys49 phospholipase A2 homologues are abundant myotoxic components in viperid ...

Snake Venomics of the Lesser Antillean Pit Vipers Bothrops caribbaeus and Bothrops lanceolatus: Correlation with Toxicological Activities and Immunoreactivity of a Heterologous Antivenom

Gutiérrez, José María; Sanz, Libia; Escolano, José; Fernández Ulate, Julián; Lomonte, Bruno; Angulo Ugalde, Yamileth; Rucavado Romero, Alexandra; Warrell, David A.; Calvete Chornet, Juan José (2008-10)

The venom proteomes of the snakes Bothrops caribbaeus and Bothrops lanceolatus, endemic to the Lesser Antillean islands of Saint Lucia and Martinique, respectively, were characterized by reversephase HPLC fractionation, ...