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Snake venomics of the South and Central American Bushmasters. Comparison of the toxin composition of Lachesis muta gathered from proteomic versus transcriptomic analysis

Sanz, Libia; Escolano, José; Ferretti, Massimo; Biscoglio, Mirtha J.; Rivera, Elena; Crescenti, Ernesto J.; Angulo Ugalde, Yamileth; Lomonte, Bruno; Gutiérrez, José María; Calvete Chornet, Juan José (2008-04-30)

We report the proteomic characterization of the venoms of two closely related pit vipers of the genus Lachesis, L. muta (South American Bushmaster) and L. stenophrys (Central American Bushmaster), and compare the toxin ...

Preclinical assessment of a polyspecific antivenom against the venoms of Cerrophidion sasai, Porthidium nasutum and Porthidium ophryomegas: Insights from combined antivenomics and neutralization assays

Gutiérrez, José María; Tsai, Wan-Chih; Pla Ferrer, Davinia; Solano Trejos, María Gabriela; Lomonte, Bruno; Sanz, Libia; Angulo Ugalde, Yamileth; Calvete Chornet, Juan José (2013-03-15)

A polyspecific antivenom is used in Central America for the treatment of envenomings by viperid snakes. This antivenom is generated in horses hyperimmunized with a mixture of venoms from Bothrops asper, Crotalus simus and ...

Venom, venomics, antivenomics

Calvete Chornet, Juan José; Sanz, Libia; Angulo Ugalde, Yamileth; Lomonte, Bruno; Gutiérrez, José María (2009)

Venoms comprise mixtures of peptides and proteins tailored by Natural Selection to act on vital systems of the prey or victim. Here we review our proteomic protocols for uncoiling the composition, immunological profile, ...

Antivenomics of Atropoides mexicanus and Atropoides picadoi snake venoms: Relationship to the neutralization of toxic and enzymatic activities

Antúnez Oliva, José; Fernández Ulate, Julián; Lomonte, Bruno; Angulo Ugalde, Yamileth; Sanz, Libia; Pérez, Alicia; Calvete Chornet, Juan José; Gutiérrez, José María (2010-09-30)

Viperid snakes of the genus Atropoides are distributed in Mexico and Central America and, owing to their size and venom yield, are capable of provoking severe envenomings in humans. This study evaluated, using an ‘antivenomics’ ...

Snake Venomics and Antivenomics of the Arboreal Neotropical Pitvipers Bothriechis lateralis and Bothriechis schlegelii

Lomonte, Bruno; Escolano, José; Fernández Ulate, Julián; Sanz, Libia; Angulo Ugalde, Yamileth; Gutiérrez, José María; Calvete Chornet, Juan José (2008-04-30)

We report the comparative proteomic characterization of the venoms of two related neotropical arboreal pitvipers from Costa Rica of the genus Bothriechis, B. lateralis (side-striped palm pit viper) and B. schlegelii (eyelash ...

Profiling the venom gland transcriptomes of Costa Rican snakes by 454 pyrosequencing

Durban, Jordi; Juárez, Paula; Angulo Ugalde, Yamileth; Lomonte, Bruno; Flores Díaz, Marietta; Alape Girón, Alberto; Sasa Marín, Mahmood; Sanz, Libia; Gutiérrez, José María; Dopazo, Joaquín; Conesa, Ana; Calvete Chornet, Juan José (BMC Genomics, 12:259, 2011, 2011)

Background: A long term research goal of venomics, of applied importance for improving current antivenom therapy, but also for drug discovery, is to understand the pharmacological potential of venoms. Individually ...

Venomic and antivenomic analyses of the Central American coral snake, Micrurus nigrocinctus (Elapidae)

Fernández Ulate, Julián; Alape Girón, Alberto; Angulo Ugalde, Yamileth; Sanz, Libia; Gutiérrez, José María; Calvete Chornet, Juan José; Lomonte, Bruno (2011-01-31)

The proteome of the venom of Micrurus nigrocinctus (Central American coral snake) was analyzed by a “venomics” approach. Nearly 50 venom peaks were resolved by RP-HPLC, revealing a complex protein composition. Comparative ...

Snake venomics and antivenomics: Proteomic tools in the design and control of antivenoms for the treatment of snakebite envenoming

Gutiérrez, José María; Lomonte, Bruno; León Montero, Guillermo; Alape Girón, Alberto; Flores Díaz, Marietta; Sanz, Libia; Angulo Ugalde, Yamileth; Calvete Chornet, Juan José (2009-03-06)

Snakebite envenoming represents a neglected tropical disease that has a heavy public health impact, particularly in Asia, Africa and Latin America. A global initiative, aimed at increasing antivenom production and ...

Snake population venomics and antivenomics of Bothrops atrox: Paedomorphism along its transamazonian dispersal and implications of geographic venom variability on snakebite management

Calvete Chornet, Juan José; Sanz, Libia; Pérez, Alicia; Borges, Adolfo; Vargas, Alba M.; Lomonte, Bruno; Angulo Ugalde, Yamileth; Gutiérrez, José María; Chalkidis, Hipócrates M.; Mourão, Rosa H.V.; Furtado, María de Fátima; Moura Da Silva, Ana M. (2011-04-11)

We describe two geographically differentiated venom phenotypes across the wide distribution range of Bothrops atrox, from the Colombian Magdalena Medio Valley through Puerto Ayacucho and El Paují, in the Venezuelan States ...

Snake Venomics of Central American Pitvipers: Clues for Rationalizing the Distinct Envenomation Profiles of Atropoides nummifer and Atropoides picadoi

Angulo Ugalde, Yamileth; Escolano, José; Lomonte, Bruno; Gutiérrez, José María; Sanz, Libia; Calvete Chornet, Juan José (2008)

We report the proteomic characterization of the Central American pitvipers Atropoides nummifer and Atropoides picadoi. The crude venoms were fractionated by reverse-phase high-performance liquid chromatography (HPLC), ...