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Rapid and sensitive detection of Shigella sonnei in feces by the use of an O-antigen-specific monoclonal antibody in a combined immunomagnetic separation-polymerase chain reaction assay
OBJECTIVE: The aim of the present work was to develop a rapid, specific and highly sensitive diagnostic method for the detection of Shigella sonnei directly from stool samples. DESIGN AND METHODS: An immunomagnetic ...
Serum Antibody Titres to Shigella Lipopolysaccharides and Invasion Plasmid Antigens in Healthy Costa Rican and Swedish Women
Specific serum antibody titres to defined lipopolysaccharides (LPS) of Shigella spp. and Salmonella serogroup B (BO) and Shigella invasion plasmid antigens (Ipa) were determined by enzyme immunoassays in healthy Costa Rican ...
Immunomagnetic separation and PCR detection show Shigellae to be common faecal agents in children from urban marginal communities of Costa Rica
Culture and immunomagnetic separation-polymerase chain reaction assays (IMS-PCR) were used to isolate and identify Shigella flexneri, S. dysenteriae type I and S. sonnei in faeces from 250 children up to 5 years and from ...