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dc.creatorBorkow, Gadi
dc.creatorGutiérrez, José María
dc.creatorOvadia, Michael
dc.date.accessioned2016-11-11T14:32:36Z
dc.date.available2016-11-11T14:32:36Z
dc.date.issued1993-09
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/29227
dc.description.abstractThree hemorrhagic factors (BaH1, BH2 and BH3) were isolated from the venom of Bothrops asper by gel filtration on Sephacryl S-200, DEAE-Sepharose chromatography, metal chelate affinity chromatography and hydrophobic interaction chromatography. They contain 55% of the total hemorrhagic activity of the whole venom when they are mixed, but lose almost half of the activity if they are separated, indicating a synergism between the three. The main hemorrhagin is BaH1 (Bothrops asper hemorrhagin 1); the other two are weak hemorrhagins but contribute to the synergism. They are acidic proteins with a pI of 4.5, 5.2 and 5; their mol. wt is 64,000, 26,000 and 55,000 respectively. The minimal hemorrhagic dose (MHD) of BaH1, BH2 and BH3 is 0.18, 2 and 1.6 micrograms, with a specific activity 55, 5 and 6.25 higher than that of the whole venom. The hemorrhagic activity of all three factors was inhibited by EDTA and ortho-phenathroline, indicating that the hemorrhagic activity is metal dependent. Phosphoramidon, soybean trypsin inhibitor, PMSF, pepstatin and aprotinin did not affect the hemorrhagic activity of the isolated factors.es_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 31, Número 9, 1993es_ES
dc.subjectAminosalicylic Acides_ES
dc.subjectAmmonium Sulfatees_ES
dc.subjectAnimalses_ES
dc.subjectAntibody Specificityes_ES
dc.subjectAntiveninses_ES
dc.subjectChromatography, Geles_ES
dc.subjectChromatography, Ion Exchangees_ES
dc.subjectCross Reactionses_ES
dc.subjectCrotalid Venomses_ES
dc.subjectDrug Synergismes_ES
dc.subjectEndopeptidaseses_ES
dc.subjectEnzyme Inhibitorses_ES
dc.subjectHemorrhagees_ES
dc.subjectHydrogen-Ion Concentrationes_ES
dc.subjectImmunodiffusiones_ES
dc.subjectImmunoelectrophoresises_ES
dc.subjectIsoelectric Focusinges_ES
dc.subjectMicees_ES
dc.subjectMolecular Weightes_ES
dc.titleIsolation and characterization of synergistic hemorrhagins from the venom of the snake Bothrops asperes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/0041-0101(93)90129-7
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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