Show simple item record

dc.creatorDíaz Oreiro, Cecilia
dc.creatorAlape Girón, Alberto
dc.creatorLomonte, Bruno
dc.creatorOlamendi Portugal, Timoteo
dc.creatorGutiérrez, José María
dc.date.accessioned2016-11-11T15:13:37Z
dc.date.available2016-11-11T15:13:37Z
dc.date.issued1994-08
dc.identifier.issn0003-9861
dc.identifier.urihttps://hdl.handle.net/10669/29232
dc.description.abstractBothrops asper myotoxin II was cleaved with cyanogen bromide to determine the role of NH2-terminal amino acid residues in its ability to destabilize negatively charged liposomes and to induce myonecrosis. After treatment, cleaved toxin was separated from its NH2-terminal octapeptide by reversed-phase HPLC. Cleaved myotoxin II lost its capability to disrupt negatively charged liposomes, whereas it maintained approximately one-third of its muscle-damaging effect. No gross antigenic changes were detected after cleavage, as judged by immunoreactivity with polyclonal sera and a set of monoclonal antibodies. However, two of the tested MAbs showed a decreased binding to CB-myotoxin II. We conclude that the NH2-terminal octapeptide has an important role in the membrane-destabilizing activity of this toxin. This domain might directly participate in the binding of toxin to membranes, as well as in its pharmacological activities. Alternatively, conformational changes might occur in cleaved protein, altering its cytotoxic effects by indirectly modifying other important domains.es_ES
dc.language.isoen_USes_ES
dc.sourceArchives of Biochemistry and Biophysics; Volumen 312, Número 2, 1994es_ES
dc.subjectAnimalses_ES
dc.subjectCreatine Kinasees_ES
dc.subjectCrotalid Venomses_ES
dc.subjectCyanogen Bromidees_ES
dc.subjectGroup II Phospholipases A2es_ES
dc.subjectLiposomeses_ES
dc.subjectMembraneses_ES
dc.subjectNeurotoxinses_ES
dc.subjectOligopeptideses_ES
dc.subjectPhospholipases Aes_ES
dc.subjectPhospholipases A2es_ES
dc.subjectReptilian Proteinses_ES
dc.subjectViperidaees_ES
dc.subjectSnake venomes_ES
dc.titleCleavage of the NH2-Terminal Octapeptide of Bothrops asper Myotoxic Lysine-49 Phospholipase A2 Reduces Its Membrane-Destabilizing Effectes_ES
dc.typeartículo científicoes_ES
dc.identifier.doi10.1006/abbi.1994.1317
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Medicina::Escuela de Medicinaes_ES


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record