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dc.creatorJiménez Álvarez, Natalia
dc.creatorEscalante Muñoz, Teresa
dc.creatorGutiérrez, José María
dc.creatorRucavado Romero, Alexandra
dc.date.accessioned2016-11-18T20:55:11Z
dc.date.available2016-11-18T20:55:11Z
dc.date.issued2008-10
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0022202X15336356es_ES
dc.identifier.issn0022-202X
dc.identifier.urihttp://hdl.handle.net/10669/29264
dc.description.abstractViperid snakebite envenomation induces blistering and dermonecrosis. The pathological alterations induced by a snake venom metalloproteinase in the skin were investigated in a mouse ear model. Metalloproteinase BaP1, from Bothrops asper, induced rapid edema, hemorrhage, and blistering; the latter two effects were abrogated by preincubation with the metalloproteinase inhibitor batimastat. Neutrophils did not play a role in the pathology, as depletion of these cells resulted in a similar histological picture. Blisters are likely to result from the direct proteolytic activity of BaP1 of proteins at the dermal-epidermal junction, probably at the lamina lucida, as revealed by immunostaining for type IV collagen and laminin. Widespread apoptosis of keratinocytes was detected by the TUNEL assay, whereas no apoptosis of capillary endothelial cells was observed. BaP1 induced a drastic reduction in the microvessel density, revealed by immunostaining for the endothelial marker vascular endothelial growth factor receptor-2. This was followed by a rapid angiogenic response, leading to a partial revascularization. Skin damage was followed by inflammation and granulation tissue formation. Then, a successful re-epithelization process occurred, and the skin of the ear regained its normal structure by 2 weeks. Venom metalloproteinase-induced skin damage reproduces the pathological changes described in snakebitten patients.es_ES
dc.language.isoen_USes_ES
dc.sourceJournal of Investigative Dermatology; Volumen 128, Número 10. 2008es_ES
dc.subjectAnimalses_ES
dc.subjectBasement Membranees_ES
dc.subjectBlisteres_ES
dc.subjectBlood Vesselses_ES
dc.subjectDermatitis, Contactes_ES
dc.subjectDisease Models, Animales_ES
dc.subjectEares_ES
dc.subjectEpitheliumes_ES
dc.subjectGranulation Tissuees_ES
dc.subjectHemorrhagees_ES
dc.subjectImmunohistochemistryes_ES
dc.subjectIn Situ Nick-End Labelinges_ES
dc.subjectMetalloendopeptidaseses_ES
dc.subjectMicees_ES
dc.subjectInbred Strainses_ES
dc.subjectMicrocirculationes_ES
dc.subjectSkines_ES
dc.subjectWound Healinges_ES
dc.titleSkin Pathology Induced by Snake Venom Metalloproteinase: Acute Damage, Revascularization, and Re-epithelization in a Mouse Ear Modeles_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1038/jid.2008.118
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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