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dc.creatorMoreira, Vanessa
dc.creatorGutiérrez, José María
dc.creatorSoares, Andreimar Martins
dc.creatorZamunér, Stella Regina
dc.creatorPurgatto, Eduardo
dc.creatorTeixeira, Catarina de Fátima
dc.date.accessioned2016-11-22T13:56:06Z
dc.date.available2016-11-22T13:56:06Z
dc.date.issued2008-09-01
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0041010108003929
dc.identifier.issn0041-0101
dc.identifier.urihttps://hdl.handle.net/10669/29310
dc.description.abstractThe effects of myotoxin III (MT-III), a phospholipase A2 (sPLA2) from Bothrops asper snake venom, and crotoxin B (CB), a neurotoxic and myotoxic sPLA2 from the venom of Crotalus durissus terrificus, on cyclooxygenases (COXs) expression and biosynthesis of prostaglandins (PGs) were evaluated, together with the mechanisms involved in these effects. Upon intraperitoneal injection in mice, both sPLA2s promoted the synthesis of PGD2 and PGE2, with a different time-course. MT-III, but not CB, induced COX-2 expression by peritoneal leukocytes without modification on COX-1 constitutive expression, whereas CB increased the constitutive activity of COX-1. MT-III increased the enzymatic activity of COX-1 and COX-2. Similar effects were observed when these sPLA2s were incubated with isolated macrophages, evidencing a direct effect on these inflammatory cells. Moreover, both toxins elicited the release of arachidonic acid from macrophages in vitro. Inhibition of cPLA2 by AACOCF3, but not of iPLA2 by PACOCF3 or BEL, significantly reduced PGD2, PGE2 and arachidonic acid (AA) release promoted by MT-III. These inhibitors did not affect MT-III-induced COX-2 expression. In contrast, cPLA2 inhibition did not modify the effects of CB, whereas iPLA2 inhibition reduced PGD2 and AA production induced by CB. These findings imply that distinct regulatory mechanisms leading to PGs' synthesis are triggered by these snake venom sPLA2s. Such differences are likely to explain the dissimilar patterns of inflammatory reaction elicited by these sPLA2s in vivo.es_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 52, Número 3. 2008es_ES
dc.subjectVenom phospholipase A2es_ES
dc.subjectCrotoxin Bes_ES
dc.subjectArachidonic acides_ES
dc.subjectProstaglandinses_ES
dc.subjectCyclooxygenaseses_ES
dc.subjectcPLA2es_ES
dc.subjectiPLA2es_ES
dc.subjectMacrophageses_ES
dc.subjectSnake venomes_ES
dc.titleSecretory phospholipases A2 isolated from Bothrops asper and from Crotalus durissus terrificus snake venoms induce distinct mechanisms for biosynthesis of prostaglandins E2 and D2 and expression of cyclooxygenaseses_ES
dc.typeartículo científico
dc.identifier.doi10.1016/j.toxicon.2008.06.012
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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