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dc.creatorSolano Trejos, María Gabriela
dc.creatorSegura Ruiz, Álvaro
dc.creatorHerrera Vega, María
dc.creatorGómez Argüello, Aarón
dc.creatorVillalta Arrieta, Mauren
dc.creatorGutiérrez, José María
dc.creatorLeón Montero, Guillermo
dc.date.accessioned2016-11-28T14:56:48Z
dc.date.available2016-11-28T14:56:48Z
dc.date.issued2010-09
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S1045105610001181es_ES
dc.identifier.issn1045-1056
dc.identifier.urihttp://hdl.handle.net/10669/29319
dc.description.abstractThe lethality neutralization assay performed in mice is the standard recommended by the World Health Organization to estimate antivenom potency. The interpretation of its results without considering its analytical capacity may lead to erroneous conclusions. Therefore, laboratories that manufacture or control antivenoms must demonstrate the appropriateness of their models. A study of the method used at Instituto Clodomiro Picado, Costa Rica, to estimate the potency of antivenoms against Bothrops asper snake venom was performed. Results show that venom doses ranging from 2 to 6 Median Lethal Doses (LD50) are appropriate to be used as challenge in this test. Variables such as the injection route, number of mice used per venom/antivenom level, and weight of the animals are critical in the estimation of the Median Effective Dose (ED50), whereas incubation time is not. The assay has an acceptable selectivity, linearity, and limits of detection and quantification. Accuracy of the lethality neutralization assay, expressed as percentage recovery, was between 71% and 127%. Intermediate precision, expressed as relative standard deviation, was ≤17%. It is concluded that the analytical characteristics of this assay are adequate enough to prove product compliance and to have statistical control over an industrial line of antivenom serial production.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A9-003]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceBiologicals; Volumen 38, Número 5. 2010es_ES
dc.subjectSnake venomes_ES
dc.subjectAntivenom potencyes_ES
dc.subjectLethalityes_ES
dc.subjectMedian lethal dosees_ES
dc.subjectLD50es_ES
dc.subjectLethality neutralization assayes_ES
dc.subjectMedian effective dosees_ES
dc.subjectED50es_ES
dc.subjectBiological assayes_ES
dc.subjectBothrops asperes_ES
dc.titleStudy of the design and analytical properties of the lethality neutralization assay used to estimate antivenom potency against Bothrops asper snake venomes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.biologicals.2010.05.006
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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