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dc.creatorCalvete Chornet, Juan José
dc.creatorSanz, Libia
dc.creatorPérez, Alicia
dc.creatorBorges, Adolfo
dc.creatorVargas, Alba M.
dc.creatorLomonte, Bruno
dc.creatorAngulo Ugalde, Yamileth
dc.creatorGutiérrez, José María
dc.creatorChalkidis, Hipócrates M.
dc.creatorMourão, Rosa H.V.
dc.creatorFurtado, María de Fátima
dc.creatorMoura Da Silva, Ana M.
dc.date.accessioned2016-11-28T19:58:28Z
dc.date.available2016-11-28T19:58:28Z
dc.date.issued2011-04-11
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S1874391911000200es_ES
dc.identifier.issn1874-3919
dc.identifier.urihttp://hdl.handle.net/10669/29323
dc.description.abstractWe describe two geographically differentiated venom phenotypes across the wide distribution range of Bothrops atrox, from the Colombian Magdalena Medio Valley through Puerto Ayacucho and El Paují, in the Venezuelan States of Amazonas and Orinoquia, respectively, and São Bento in the Brazilian State of Maranhão. Colombian and Venezuelan venoms show an ontogenetic toxin profile phenotype whereas Brazilian venoms exhibit paedomorphic phenotypes. Venoms from each of the 16 localities sampled contain both population-specific toxins and proteins shared by neighboring B. atrox populations. Mapping the molecular similarity between conspecific populations onto a physical map of B. atrox range provides clues for tracing dispersal routes that account for the current biogeographic distribution of the species. The proteomic pattern is consistent with a model of southeast and southwest dispersal and allopatric fragmentation northern of the Amazon Basin, and trans-Amazonian expansion through the Andean Corridor and across the Amazon river between Monte Alegre and Santarém. An antivenomic approach applied to assess the efficacy towards B. atrox venoms of two antivenoms raised in Costa Rica and Brazil using Bothrops venoms different than B. atrox in the immunization mixtures showed that both antivenoms immunodepleted very efficiently the major toxins (PIII-SVMPs, serine proteinases, CRISP, LAO) of paedomorphic venoms from Puerto Ayacucho (Venezuelan Amazonia) through São Bento, but had impaired reactivity towards PLA2 and P-I SVMP molecules abundantly present in ontogenetic venoms. The degree of immunodepletion achieved suggests that each of these antivenoms may be effective against envenomations by paedomorphic, and some ontogenetic, B. atrox venoms.es_ES
dc.description.sponsorshipMinisterio de Ciencia e Innovación/[BFU2007-61563]/MICINN/Españaes_ES
dc.description.sponsorshipMinisterio de Ciencia e Innovación/[BFU2010-1737]/MICINN/Españaes_ES
dc.description.sponsorshipInstituto Nacional de Ciências e Tecnologia em Toxinas//INCTTOX/Brasiles_ES
dc.description.sponsorshipGeneralitat Valenciana/[PROMETEO/2010/005]//Españaes_ES
dc.description.sponsorshipConsejo Superior de Investigaciones Científicas/2009CR0021/CRUSA-CSI/Españaes_ES
dc.language.isoen_USes_ES
dc.sourceJournal of the America Chemical Society; Volumen 74, Número 4. 2011es_ES
dc.subjectBothrops atroxes_ES
dc.subjectSnake venom proteomicses_ES
dc.subjectSnake population venomicses_ES
dc.subjectGeographic venom variabilityes_ES
dc.subjectPaedomorpohismes_ES
dc.subjectN-terminal sequencees_ES
dc.subjectMass spectrometryes_ES
dc.titleSnake population venomics and antivenomics of Bothrops atrox: Paedomorphism along its transamazonian dispersal and implications of geographic venom variability on snakebite managementes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.jprot.2011.01.003
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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