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dc.creatorRucavado Romero, Alexandra
dc.creatorLomonte, Bruno
dc.creatorOvadia, Michael
dc.creatorGutiérrez, José María
dc.date.accessioned2016-12-09T16:13:37Z
dc.date.available2016-12-09T16:13:37Z
dc.date.issued1995-12
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0014480085710428es_ES
dc.identifier.issn0014-4800
dc.identifier.urihttp://hdl.handle.net/10669/29379
dc.description.abstractThe pathogenesis of hemorrhage and other local effects induced by the metalloproteinase BaP1, isolated from Bothrops asper venom, was investigated using various in vivo and in vitro models. Upon intramuscular injection in mice BaP1 caused rapid hemorrhage in muscular and adipose tissues. Vital microscopy using mouse cremaster muscle evidenced the formation of multiple hemorrhagic foci of an explosive character, originating from capillaries and small venules. In contrast to crude B. asper venom, which besides hemorrhage also induced myonecrosis and thrombosis, vital microscopy detected only hemorrhage after application of BaP1, during the 40-min observation period. However, histological observation in mouse gastrocnemius muscle evidenced a few areas of limited myonecrosis was followed by an incomplete regenerative response, since regenerating muscle fibers were interspersed with fibrosis in some areas. Metalloproteinase BaP1 was not cytotoxic to human and murine endothelial cells in culture, causing only a mild detachment from the culture plate. BaP1 hydrolyzed types I and IV collagen, fibronectin, and laminin upon incubation with these extracellular matrix proteins in vitro. These results suggest that hemorrhage induced by BaP1 is due primarily to the proteolytic degradation to basement membrane components of microvessels and that endothelial cell disruption may be a secondary event. It is concluded that, in addition to hemorrhage, BaP1 contributes to the local tissue damage caused by the venom by inducing myonecrosis, inflammation, and extracellular matrix alterations.es_ES
dc.language.isoen_USes_ES
dc.sourceExperimental and Molecular Pathology; Volumen 63, Número 3, 1995es_ES
dc.subjectAnimalses_ES
dc.subjectBothropses_ES
dc.subjectCreatine Kinasees_ES
dc.subjectCrotalid Venomses_ES
dc.subjectEndothelium, Vasculares_ES
dc.subjectExtracellular Matrix Proteinses_ES
dc.subjectMalees_ES
dc.subjectMetalloendopeptidaseses_ES
dc.subjectMicees_ES
dc.subjectMuscleses_ES
dc.subjectSnake venom
dc.titleLocal tissue damage induced by BaP1, a metalloproteinase isolated from Bothrops asper (Terciopelo) snake venomes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1006/exmp.1995.1042
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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