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dc.creatorHerrera Vega, María
dc.creatorLeón Montero, Guillermo
dc.creatorSegura Ruiz, Álvaro
dc.creatorMeneses, Fabricio
dc.creatorLomonte, Bruno
dc.creatorChippaux, Jean Philippe
dc.creatorGutiérrez, José María
dc.date.accessioned2016-12-15T18:25:11Z
dc.date.available2016-12-15T18:25:11Z
dc.date.issued2005-12
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/29409
dc.description.abstractCaprylic acid purification of IgG, currently used in the manufacture of horse-derived antivenoms, was successfully adapted for the preparation of sheep and camel IgG. Sheep IgG had a molecular mass of approximately 150 kDa, whereas camel IgG presented two bands of molecular masses of approximately 160 and 100 kDa, the latter corresponding to heavy-chain IgG, which is devoid of light chains. Horse, sheep and camel IgGs were compared by several parameters aiming at predicting their potential for induction of early and late adverse reactions. Horse and sheep IgGs showed a higher anticomplementary activity than camel IgG, and also elicited a higher anti-IgG response than camel IgG, when injected in mice. Horse IgG agglutinated human type O+ erythrocytes, whereas no such reactivity was observed in sheep and camel IgG preparations. A novel procedure was used for the detection of antibodies in human serum against animal IgGs. It was found that a pool of human sera collected in Costa Rica had a higher titer of antibodies directed against horse and sheep IgGs than against camel IgG. Overall, camel IgG showed the lowest potential for the induction of adverse reactions among the three IgGs tested.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A4-503]/UCR/Costa Ricaes_ES
dc.description.sponsorship/[883.701-3]/UNESCO/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 46, Número 7. 2005es_ES
dc.subjectIgGes_ES
dc.subjectCaprylic Acides_ES
dc.subjectAnticomplementary Activityes_ES
dc.subjectAntivenomes_ES
dc.subjectAdverse Reactionses_ES
dc.subjectAnti-IgG Antibodieses_ES
dc.subjectSnake venomes_ES
dc.titleFactors associated with adverse reactions induced by caprylic acid-fractionated whole IgG preparations: comparison between horse, sheep and camel IgGses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.toxicon.2005.08.004
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-A4-503


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