A Lys49 phospholipase A2 homologue from Bothrops asper snake venom induces proliferation, apoptosis and necrosis in a lymphoblastoid cell line
Mora Rodríguez, Rodrigo
Valverde Rojas, Berta E.
Díaz Oreiro, Cecilia
Gutiérrez, José María
MetadataShow full item record
Lys49 phospholipase A2 homologues are abundant in viperid snake venoms. These proteins have substitutions at the calcium-binding loop and catalytic center which render them enzymatically inactive; however, they display a series of toxic activities, particularly cytotoxicity upon various cell lines in vitro. In this study we explored whether myotoxin II (MT-II), a Lys49 phospholipase A2 homologue from the venom of the snake Bothrops asper, is capable of inducing various effects in a single cell type, using the lymphoblastoid B cell line CRL-8062 as a model. Cells were incubated with varying concentrations of MT-II for 24 and 48 h, time intervals that are more prolonged than the usual incubation times previously used in the characterization of this toxin. Results indicate that MT-II induces proliferation at low concentrations (0.5–5.0 μg/mL). Apoptosis was predominant at higher toxin levels (5–25 μg/mL), whereas necrosis, associated with overt plasma membrane disruption, occurred at concentrations ≥25 μg/mL, and was the predominant effect at higher MT-II concentrations (50 μg/mL). It is concluded that a single phospholipase A2 homologue can induce markedly different effects on a single cell line, depending on the concentration used, an observation that may have implications for the action of this type of venom component in vivo.
External link to the item10.1016/j.toxicon.2005.01.008
- Microbiología