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dc.creatorMora Rodríguez, Rodrigoes_ES
dc.creatorValverde Rojas, Berta E.es_ES
dc.creatorDíaz Oreiro, Ceciliaes_ES
dc.creatorLomonte, Brunoes_ES
dc.creatorGutiérrez, José Maríaes_ES
dc.date.accessioned2017-01-16T17:44:11Zes_ES
dc.date.available2017-01-16T17:44:11Zes_ES
dc.date.issued2005-04es_ES
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0041010105000206es_ES
dc.identifier.issn0041-0101es_ES
dc.identifier.urihttp://hdl.handle.net/10669/29424es_ES
dc.description.abstractLys49 phospholipase A2 homologues are abundant in viperid snake venoms. These proteins have substitutions at the calcium-binding loop and catalytic center which render them enzymatically inactive; however, they display a series of toxic activities, particularly cytotoxicity upon various cell lines in vitro. In this study we explored whether myotoxin II (MT-II), a Lys49 phospholipase A2 homologue from the venom of the snake Bothrops asper, is capable of inducing various effects in a single cell type, using the lymphoblastoid B cell line CRL-8062 as a model. Cells were incubated with varying concentrations of MT-II for 24 and 48 h, time intervals that are more prolonged than the usual incubation times previously used in the characterization of this toxin. Results indicate that MT-II induces proliferation at low concentrations (0.5–5.0 μg/mL). Apoptosis was predominant at higher toxin levels (5–25 μg/mL), whereas necrosis, associated with overt plasma membrane disruption, occurred at concentrations ≥25 μg/mL, and was the predominant effect at higher MT-II concentrations (50 μg/mL). It is concluded that a single phospholipase A2 homologue can induce markedly different effects on a single cell line, depending on the concentration used, an observation that may have implications for the action of this type of venom component in vivo.es_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 45, Número 5, 2005es_ES
dc.subjectLys49 Phospholipase A2 Homologuees_ES
dc.subjectBothrops Asperes_ES
dc.subjectApoptosises_ES
dc.subjectNecrosises_ES
dc.subjectProliferationes_ES
dc.subjectSnake venomes_ES
dc.titleA Lys49 phospholipase A2 homologue from Bothrops asper snake venom induces proliferation, apoptosis and necrosis in a lymphoblastoid cell linees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.toxicon.2005.01.008es_ES
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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