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dc.creatorRucavado Romero, Alexandraes_ES
dc.creatorNúñez, Javieres_ES
dc.creatorGutiérrez, José Maríaes_ES
dc.date.accessioned2017-01-20T17:58:05Zes_ES
dc.date.available2017-01-20T17:58:05Zes_ES
dc.date.issued1998-08es_ES
dc.identifier.issn1365-2613es_ES
dc.identifier.urihttp://hdl.handle.net/10669/29445es_ES
dc.description.abstractBlister formation and skin damage can be induced by BaP1, a haemorrhagic metalloproteinase from the venom of the snake Bothrops asper. Pathological changes in the skin were investigated after intramuscular injections of Bothrops asper haemorrhagic metalloproteinase BaP1. Blisters developed within the first hour, with separation of epidermis from the dermal-epidermal junction, whereas acantholysis of epithelial cells was not observed. After the third hour there was ulceration with formation of a proteinaceous scab and inflammatory infiltrate. By 7 to 14 days there was evidence of a regenerative process in dermis and epidermis. Haemorrhage occurred in both dermis and hypodermis as a consequence of BaP1 injection, together with damage of sebaceous glands and an inflammatory reaction in which enlarged macrophages were the predominant cell type. Zymography assays showed the presence of several endogenous metalloproteinases in the exudate, skin homogenates and plasma. In addition, BaP1 was detected in exudates and plasma by immunoblotting. This technique also demonstrated the presence of components immunologically related to laminin and collagen type IV in exudates. It is suggested that BaP1, and probably endogenous matrix metalloproteinases, degrade some protein components at the dermal-epidermal junction, inducing the formation of blisters.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-96-262]/UCR/Costa Ricaes_ES
dc.description.sponsorshipInternational Foundation for Science/[F/2707-1]/IFS/Sueciaes_ES
dc.language.isoen_USes_ES
dc.sourceInternational Journal of Experimental Pathology. Volumen 79, Número 4, 1998es_ES
dc.subjectAnimalses_ES
dc.subjectBlisteres_ES
dc.subjectBothropses_ES
dc.subjectCrotalid Venomses_ES
dc.subjectElectrophoresis, Polyacrylamide Geles_ES
dc.subjectExtracellular Matrix Proteinses_ES
dc.subjectExudates and Transudateses_ES
dc.subjectGelatinaseses_ES
dc.subjectImmunoblottinges_ES
dc.subjectMetalloendopeptidaseses_ES
dc.subjectMicees_ES
dc.subjectSkin Diseaseses_ES
dc.titleBlister formation and skin damage induced by BaP1, a haemorrhagic metalloproteinase from the venom of the snake Bothrops asperes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-96-262es_ES


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