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dc.creatorFranceschi, Aida
dc.creatorRucavado Romero, Alexandra
dc.creatorMora, Nidia
dc.creatorGutiérrez, José María
dc.date.accessioned2017-01-24T16:49:39Z
dc.date.available2017-01-24T16:49:39Z
dc.date.issued2000-01
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0041010199001270es_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/29459
dc.description.abstractA hemorrhagic metalloproteinase, named BaH4, was isolated from the venom of the snake Bothrops asper by a combination of ion-exchange chromatography on DEAE-Sepharose and gel filtration on Sephacryl S-200. BaH4 is a 69 kDa protein with a pI of 5.3. It was recognized by antibodies raised against hemorrhagic metalloproteinase BaH1 isolated from B. asper venom, with a reaction of partial immunologic identity. BaH4 shows proteolytic activity on biotinylated casein, hide powder azure and fibrin, although having lower activity than crude B. asper venom and metalloproteinase BaP1 isolated from the same venom. BaH4 hydrolyzed fibronectin, laminin and type IV collagen in vitro, albeit at a relatively high enzyme:substrate ratio. Proteolytic activity was inhibited by chelating agents and 2-mercaptoethanol, but not by soybean trypsin inhibitor. Prominent hemorrhage developed in gastrocnemius and cremaster muscles after administration of BaH4. Moreover, it induced lethality in mice after intravenous injection, with an LD50 of 0.37 μg/g. Histological observations showed conspicuous pulmonary hemorrhage when the enzyme was injected intravenously. BaH4 is a hemorrhagic metalloproteinase which may play a relevant role in local and systemic bleeding characteristic of B. asper envenomations.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-98-202]/UCR/Costa Ricaes_ES
dc.description.sponsorshipInternational Foundation for Science/[F/2707-1]/IFS/Sueciaes_ES
dc.description.sponsorshipDeutscher Akademischer Austauschdienst//DAAD/Alemaniaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 38, Número 1, 2000es_ES
dc.subjectHemorrhagees_ES
dc.subjectMetalloproteinasees_ES
dc.subjectSnake venomes_ES
dc.subjectBothrops asperes_ES
dc.titlePurification and characterization of BaH4, a hemorrhagic metalloproteinase from the venom of the snake Bothrops asperes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/S0041-0101(99)00127-0
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-98-202


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