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dc.creatorEscalante Muñoz, Teresa
dc.creatorFranceschi, Aida
dc.creatorRucavado Romero, Alexandra
dc.creatorGutiérrez, José María
dc.date.accessioned2017-01-25T14:56:44Z
dc.date.available2017-01-25T14:56:44Z
dc.date.issued2000-07-15
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0006295200003026es_ES
dc.identifier.issn0006-2952
dc.identifier.urihttp://hdl.handle.net/10669/29466
dc.description.abstractBatimastat (BB-94), a synthetic hydroxamate peptidomimetic matrix metalloproteinase inhibitor, was tested for its ability to inhibit proteolytic and toxic effects induced by BaP1, a 24-kDa hemorrhagic metalloproteinase isolated from the venom of Bothrops asper, the medically most important snake species in Central America and southern Mexico. Batimastat inhibited proteolytic activity on biotinylated casein, with anic50 of 80 nM. In addition, batimastat was effective in inhibiting hemorrhagic, dermonecrotic, and edema-forming activities of this metalloproteinase if incubated with the enzyme prior to the assays. When the inhibitor was administered i.m. at the site of the toxin injection without preincubation, rapidly after metalloproteinase administration, it totally abrogated the hemorrhagic and dermonecrotic effects of BaP1. Inhibition was less effective as the time lapse between toxin and batimastat injection increased, due to the extremely rapid development of BaP1-induced local tissue damage in this experimental model. On the other hand, batimastat was ineffective if administered by the i.p. route immediately after toxin injection. It is concluded that batimastat, and probably other synthetic metalloproteinase inhibitors, may become useful therapeutic tools aimed at the in situ inhibition of venom metalloproteinases, when injected at the site of the bite rapidly after envenomation.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741–98-202]/UCR/Costa Ricaes_ES
dc.description.sponsorshipInternational Foundation for Science/[F/2707–1]/IFS/Sueciaes_ES
dc.description.sponsorshipConsejo Nacional para Investigaciones Científicas y Tecnológicas/[98-012-F0]/CONICIT/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceBiochemical Pharmacology; Volumen 60, Número 2, 2000es_ES
dc.subjectMatrix metalloproteinaseses_ES
dc.subjectMetalloproteinase inhibitorses_ES
dc.subjectBatimastates_ES
dc.subjectHydroxamateses_ES
dc.subjectHemorrhagees_ES
dc.subjectVenom metalloproteinaseses_ES
dc.subjectBothrops asperes_ES
dc.subjectSnake venomes_ES
dc.titleEffectiveness of batimastat, a synthetic inhibitor of matrix metalloproteinases, in neutralizing local tissue damage induced by BaP1, a hemorrhagic metalloproteinase from the venom of the snake Bothrops asperes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/S0006-2952(00)00302-6
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-98-202
dc.identifier.pmid10825472


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