Universidad de Costa Rica
  • Sobre Kérwá
  • Acceso Abierto
  • Cómo Depositar
  • Políticas
  • Contacto
    • español
    • English
  • español 
    • español
    • English
  • Acceder
Ver ítem 
  •   Repositorio Kérwá
  • Investigación
  • Salud
  • Microbiología
  • Ver ítem
  •   Repositorio Kérwá
  • Investigación
  • Salud
  • Microbiología
  • Ver ítem
JavaScript is disabled for your browser. Some features of this site may not work without it.

Myotoxic phospholipases A2 in Bothrops snake venoms: Effect of chemical modifications on the enzymatic and pharmacological properties of bothropstoxins from Bothrops jararacussu

Artículo científico
Ver/
171_2000_Biochimie_Andriao-Escarso_Bothrops_myotoxic_PLA2s.pdf (1.063Mb)
Fecha
2000-08
Autor
Andrião Escarso, Silvia H.
Soares, Andreimar Martins
Rodrigues, Veridiana M.
Angulo Ugalde, Yamileth
Díaz Oreiro, Cecilia
Lomonte, Bruno
Gutiérrez, José María
Giglio, José Roberto
Metadatos
Mostrar el registro completo del ítem
Resumen
Venoms from eight Bothrops spp. were fractionated by ion-exchange chromatography on CM-Sepharose at pH 8.0 for the purification of myotoxins. Chromatographic profiles showed differences regarding myotoxic components among these venoms. B. alternatus, B. atrox and B. jararaca venoms did not show the major basic myotoxic fractions identified in the other venoms. Polyacrylamide gel electrophoresis for basic proteins also showed distinct patterns for these toxins. In vivo, all the isolated myotoxins induced release of creatine kinase due to necrosis of muscle fibers, accompanied by polymorphonuclear cell infiltration, and edema in the mouse paw. In addition, the toxins showed cytotoxic and liposome-disrupting activities in vitro. B. jararacussu bothropstoxins-I (BthTX-I) and II (BthTX-II) were submitted to chemical modifications of: His, by 4-bromophenacyl bromide (BPB) or photooxidation by Rose Bengal (RB); Tyr, by 2-nitrobenzenesulphonyl fluoride (NBSF); and Trp, by o-nitrophenylsulphenyl chloride (NPSC). The myotoxic and cytotoxic activities of BthTX-I, a Lys49 PLA2 homologue, after modification by BPB, RB, NBSF and NPSC, were reduced to 50%, 20%, 75%, 65% and 13%, 0.5%, 76%, 58%, respectively. However, the edema-inducing and liposome-disrupting activities were not significantly reduced by the above modifications. BPB-treated BthTX-II, an Asp49 PLA2 homologue, lost most of its catalytic, indirect hemolytic, anticoagulant, myotoxic and cytotoxic activities. The edema-inducing and liposome-disrupting activities were reduced to 50% and 80%, respectively. Lethality caused by BthTX-I and -II was strongly reduced after treatment with BPB or RB, but only partially with NBSF or NPSC. BthTX-I and -II, both native or modified, migrated similarly in a charge-shift electrophoresis. Antibodies raised against BthTX-I or -II, B. asper Basp-II and the C-terminal 115-129 peptide from Basp-II did not show significant differences in their cross-reactivity with the modified toxins, except with RB photooxidized toxins.
URI
http://hdl.handle.net/10669/29470
Enlace externo al ítem
10.1016/S0300-9084(00)01150-0
http://www.sciencedirect.com/science/article/pii/S0300908400011500
Colecciones
  • Microbiología [879]



  • Repositorios universitarios

  • Repositorio del SIBDI-UCR
  • Biblioteca Digital del CIICLA
  • Repositorio Documental Rafael Obregón Loría (CIHAC)
  • Biblioteca Digital Carlos Melendez (CIHAC)
  • Repositorio de Fotografías
  • Colección de videos de UPA-VAS
  • Sitios recomendados

  • Buscador regional de LA Referencia
  • Buscador del Open ROAR
  • Scientific Electronic Library Online (SciELO)
  • Directory of Open Access Journals (DOAJ)
  • Redalyc
  • Redes sociales

  • facebook.com/repositoriokerwa
  • @Ciencia_UCR
  • Sobre Kérwá
  • Acceso Abierto
  • Cómo depositar
  • Políticas
Contacto | Sugerencias
Repositorio Institucional de la Universidad de Costa Rica. Algunos derechos reservados. Este repositorio funciona con DSpace.
Universidad de Costa Rica
 

 

Listar

Todo KérwáComunidades & ColeccionesTítulosAutoresPalabra clavePor procedenciaPor tipoEsta colecciónTítulosAutoresPalabra clavePor procedenciaPor tipo

Mi cuenta

AccederRegistro

  • Repositorios universitarios

  • Repositorio del SIBDI-UCR
  • Biblioteca Digital del CIICLA
  • Repositorio Documental Rafael Obregón Loría (CIHAC)
  • Biblioteca Digital Carlos Melendez (CIHAC)
  • Repositorio de Fotografías
  • Colección de videos de UPA-VAS
  • Sitios recomendados

  • Buscador regional de LA Referencia
  • Buscador del Open ROAR
  • Scientific Electronic Library Online (SciELO)
  • Directory of Open Access Journals (DOAJ)
  • Redalyc
  • Redes sociales

  • facebook.com/repositoriokerwa
  • @Ciencia_UCR
  • Sobre Kérwá
  • Acceso Abierto
  • Cómo depositar
  • Políticas
Contacto | Sugerencias
Repositorio Institucional de la Universidad de Costa Rica. Algunos derechos reservados. Este repositorio funciona con DSpace.
Universidad de Costa Rica