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dc.creatorRojas Umaña, Ermila
dc.creatorSaravia Otten, Patricia
dc.creatorAngulo Ugalde, Yamileth
dc.creatorArce, Viviana
dc.creatorLomonte, Bruno
dc.creatorChávez, Juan José
dc.creatorVelásquez, Rubén
dc.creatorThelestam, Mónica
dc.creatorGutiérrez, José María
dc.date.accessioned2017-02-01T21:37:12Z
dc.date.available2017-02-01T21:37:12Z
dc.date.issued2001-07
dc.identifier.issn1532-0456
dc.identifier.urihttp://hdl.handle.net/10669/29478
dc.description.abstractA comparative study was performed on the venoms of the crotaline snake Atropoides nummifer from Guatemala and Honduras. SDS-polyacrylamide gel electrophoresis, under reducing conditions, revealed a highly similar pattern of these venoms, and between them and the venom of the same species from Costa Rica. Similar patterns were also observed in ion-exchange chromatography on CM-Shephadex C-25, in which a highly basic myotoxic fraction was present. This fraction was devoid of phospholipase A(2) activity and strongly reacted, by enzyme-immunoassay, with an antiserum against Bothrops asper myotoxin II, a Lys-49 phospholipase A(2) homologue. A basic myotoxin of 16 kDa was isolated to homogeneity from the venom of A. nummifer from Honduras, showing amino acid composition and N-terminal sequence similar to those of Lys-49 phospholipase A(2) variants previously isolated from other crotaline snake venoms. Guatemalan and Honduran A. nummifer venoms have a qualitatively similar toxicological profile, characterized by: lethal; hemorrhagic; myotoxic; edema-forming; coagulant; and defibrinating activities, although there were significant quantitative variations in some of these activities between the two venoms. Neutralization of toxic activities by two commercially-available antivenoms in the region was studied. Polyvalent antivenom produced by Instituto Clodomiro Picado was effective in the neutralization of: lethal; hemorrhagic; myotoxic; coagulant; defibrinating; and phospholipase A(2) activities, but ineffective against edema-forming activity. On the other hand, MYN polyvalent antivenom neutralized: hemorrhagic; myotoxic; coagulant; defibrinating; and phospholipase A(2) activities, albeit with a lower potency than Instituto Clodomiro Picado antivenom. MYN antivenom failed to neutralize lethal and edema-forming activities of A. nummifer venoms.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-99-269]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-89-057]/UCR/Costa Ricaes_ES
dc.description.sponsorshipNetwork for Research and Training in Tropical Diseases in Central America//NeTropica/es_ES
dc.description.sponsorshipInternational Foundation for Science/[2677-1]/IFS/Sueciaes_ES
dc.description.sponsorshipConsejo Nacional de Ciencia y Tecnología/[MS-058-1]/CONICYT/Guatemalaes_ES
dc.language.isoen_USes_ES
dc.sourceComparative Biochemistry and Physiology Part C: Toxicology & pharmacology; Volumen 129, Número 2. 2001es_ES
dc.subjectAmino Acid Sequencees_ES
dc.subjectAnimalses_ES
dc.subjectAntiveninses_ES
dc.subjectCells, Culturedes_ES
dc.subjectChromatography, High Pressure Liquides_ES
dc.subjectChromatography, Ion Exchangees_ES
dc.subjectCoagulantses_ES
dc.subjectCross Reactionses_ES
dc.subjectCrotalid Venomses_ES
dc.subjectEdemaes_ES
dc.subjectElectrophoresis, Polyacrylamide Geles_ES
dc.subjectFibrinolysises_ES
dc.subjectGuatemalaes_ES
dc.subjectHemorrhagees_ES
dc.subjectHondurases_ES
dc.subjectIn Vitro Techniqueses_ES
dc.subjectInjections, Intradermales_ES
dc.subjectInjections, Intramusculares_ES
dc.subjectInjections, Intraperitoneales_ES
dc.subjectLethal Dose 50es_ES
dc.subjectMicees_ES
dc.subjectMuscle, Skeletales_ES
dc.subjectNeutralization Testses_ES
dc.subjectPhospholipases Aes_ES
dc.subjectViperidaees_ES
dc.subjectSnake venomes_ES
dc.titleVenom of the crotaline snake Atropoides nummifer (jumping viper) from Guatemala and Honduras: comparative toxicological characterization, isolation of a myotoxic phospholipase A(2) homologue and neutralization by two antivenomses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/s1532-0456(01)00198-3
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-89-057
dc.identifier.codproyecto741-99-269
dc.identifier.pmid11423387
dc.identifier.pmid11423387


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