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dc.creatorGarcía González, Mildred
dc.creatorMonge Monge, María
dc.creatorLeón Montero, Guillermo
dc.creatorLizano González, Sergio
dc.creatorSegura, Eduardo
dc.creatorSolano Trejos, María Gabriela
dc.creatorRojas Céspedes, Gustavo
dc.creatorGutiérrez, José María
dc.date.accessioned2017-02-03T16:03:49Z
dc.date.available2017-02-03T16:03:49Z
dc.date.issued2002-06
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S1045105602903295es_ES
dc.identifier.issn1045-1056
dc.identifier.urihttp://hdl.handle.net/10669/29486
dc.description.abstractIntravenous administration of antivenoms is associated with early adverse reactions in a number of cases, but the causes of this phenomenon are still unclear. The effect of preservatives (phenol and thimerosal) on IgG aggregate and dimer formation, in vitro complement-activating effect and hypotensive activity of a whole IgG horse liquid polyvalent antivenom, produced by caprylic acid fractionation, was assessed. These parameters were studied since they have been associated with the development of early adverse reactions to the administration of antivenoms and human immunoglobulins. After a three-year storage period at 4 degrees C, antivenoms with preservatives had an increased content of IgG aggregates and dimers when compared with antivenom devoid of phenol and thimerosal. These observations correlate with a slight increment in the turbidity of preservative-containing antivenoms. The three antivenoms studied (formulation: no preservatives; with phenol and thimerosal; with thimerosal alone) activated human complement in vitro, with only minor quantitative differences among them. When antivenoms were administered as a bolus intravenous injection in rats, a rapid and prominent hypotension of short duration was observed after injection of phenol-containing antivenom, whereas such an effect was absent in antivenom free of preservative and in the one containing only thimerosal. Bolus injection of saline solution with phenol resulted in a similar hypotension, indicating that the effect is due to phenol. However, when phenol-containing antivenom was diluted 1:5 with saline solution before infusion, as occurs in the clinical use of this product, no hypotension was observed. Our results stress the need to evaluate the effects of preservatives on the physicochemical and pharmacological characteristics of antivenoms.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A1-027]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceBiologicals; Volumen 30, Número 2. 2002es_ES
dc.subjectAnimalses_ES
dc.subjectAntihypertensive Agentses_ES
dc.subjectAntiveninses_ES
dc.subjectCaprylateses_ES
dc.subjectChromatography, Geles_ES
dc.subjectComplement Activationes_ES
dc.subjectDimerizationes_ES
dc.subjectHorseses_ES
dc.subjectHumanses_ES
dc.subjectImmunoglobulin Ges_ES
dc.subjectPreservatives, Pharmaceuticales_ES
dc.subjectSnake Biteses_ES
dc.subjectTemperaturees_ES
dc.subjectTime Factorses_ES
dc.subjectSnake venomes_ES
dc.titleEffect of Preservatives on IgG Aggregation, Complement-activating Effect and Hypotensive Activity of Horse Polyvalent Antivenom Used in Snakebite Envenomationes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1006/biol.2002.0329
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-A1-027
dc.identifier.pmid12127316


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