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dc.creatorBernardes, Carolina P.
dc.creatorMenaldo, Danilo L.
dc.creatorCamacho Umaña, Erika
dc.creatorRosa, José C.
dc.creatorEscalante Muñoz, Teresa
dc.creatorRucavado Romero, Alexandra
dc.creatorLomonte, Bruno
dc.creatorGutiérrez, José María
dc.creatorSampaio, Suely V.
dc.creatorRosa, José C.
dc.date.accessioned2017-06-09T19:14:46Z
dc.date.available2017-06-09T19:14:46Z
dc.date.issued2013-03-27
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S1874391913000511es_ES
dc.identifier.issn1874-3919
dc.identifier.urihttp://hdl.handle.net/10669/30083
dc.description.abstractBothrops pirajai snake venom was analyzed by a proteomic strategy. Proteins were separated by RP-HPLC, followed by SDS-PAGE, in-gel tryptic digestion, identification by MALDI-TOF/TOF mass spectrometry, and assignment to known protein families by similarity. Proteins belonging to six families were found in B. pirajai venom, including abundant PLA2s and metalloproteinases, with the remaining proteins distributed among l-amino acid oxidase, serine proteinase, disintegrin and lectin-like families. A P-I class metalloproteinase, named BpirMP, was isolated from this venom by three chromatographic steps. The enzyme has a molecular mass of 23.1 kDa, as determined by mass spectrometry. Its proteolytic activity on azocasein was inhibited by chelating and reducing agents, with optimum activity at higher pH values and 37 °C. BpirMP presented weak hemorrhagic activity, with an MHD of 50 μg, and was able to hydrolyze basement membrane components in vivo and in vitro. The toxin cleaved both Aα and Bβ chains of fibrinogen and was also able to degrade fibrin and blood clots in vitro. The primary sequence analysis indicates that BpirMP contains a zinc ligand motif and a CVM motif that is associated with a Met-turn structure. These results demonstrate that BpirMP is a zinc-dependent hemorrhagic metalloproteinase with fibrin(ogen)olytic and thrombolytic activities.es_ES
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo//FAPESP/Brasiles_ES
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior//CAPES//Brasiles_ES
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico//CNPq/Brasiles_ES
dc.language.isoen_USes_ES
dc.sourceJournal of Proteomics; Volumen 80. 2013es_ES
dc.subjectSnake venomes_ES
dc.subjectBothrops pirajaies_ES
dc.subjectProteomees_ES
dc.subjectMetalloproteinasees_ES
dc.subjectBasement membranees_ES
dc.titleProteomic analysis of Bothrops pirajai snake venom and characterization of BpirMP, a new P-I metalloproteinasees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.jprot.2013.01.021
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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