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dc.creatorOlmedo, Hidekel
dc.creatorHerrera Vega, María
dc.creatorRojas Sánchez, Leonardo
dc.creatorVillalta Arrieta, Mauren
dc.creatorVargas Arroyo, Mariángela
dc.creatorLeiguez, Elbio
dc.creatorTeixeira, Catarina de Fátima
dc.creatorEstrada Umaña, Ricardo
dc.creatorGutiérrez, José María
dc.creatorLeón Montero, Guillermo
dc.creatorMontero Villalobos, Mavis Lili
dc.date.accessioned2017-06-12T17:36:41Z
dc.date.available2017-06-12T17:36:41Z
dc.date.issued2014
dc.identifier.citationhttp://www.tandfonline.com/doi/abs/10.3109/1547691X.2013.772267?journalCode=iimt20es_ES
dc.identifier.issn1547-691X
dc.identifier.urihttp://hdl.handle.net/10669/30092
dc.description.abstractThe adjuvanticity of aluminum hydroxide and calcium phosphate on the antibody response in mice towards the venom of the snake Bothrops asper was studied. It was found that, in vitro, most of the venom proteins are similarly adsorbed by both mineral salts, with the exception of some basic phospholipases A2, which are better adsorbed by calcium phosphate. After injection, the adjuvants promoted a slow release of the venom, as judged by the lack of acute toxicity when lethal doses of venom were administered to mice. Leukocyte recruitment induced by the venom was enhanced when it was adsorbed on both mineral salts; however, venom adsorbed on calcium phosphate induced a higher antibody response towards all tested HPLC fractions of the venom. On the other hand, co-precipitation of venom with calcium phosphate was the best strategy for increasing: (1) the capacity of the salt to couple venom proteins in vitro; (2) the venom ability to induce leukocyte recruitment; (3) phagocytosis by macrophages; and (4) a host antibody response. These findings suggest that the chemical nature is not the only one determining factor of the adjuvant activity of mineral salts.es_ES
dc.description.sponsorshipInternational Foundation for Science/[B/5043-1]/IFS/Sueciaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B2-501]/UCR/Costa Ricaes_ES
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo/[08/57898-0]/INCTTOX-CNPq/FAPESP/Brasiles_ES
dc.language.isoen_USes_ES
dc.sourceJournal of Immunotoxicology; Volume 11. 2014es_ES
dc.subjectAluminum hydroxidees_ES
dc.subjectAntibody responsees_ES
dc.subjectAnti-venomes_ES
dc.subjectCalcium phosphatees_ES
dc.subjectImmunological adjuvantes_ES
dc.subjectSnake venomes_ES
dc.titleComparison of the adjuvant activity of aluminum hydroxide and calcium phosphate on the antibody response towards Bothrops asper snake venomes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.3109/1547691X.2013.772267
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.pmid23506358


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