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dc.creatorPla Ferrer, Davinia
dc.creatorPaiva, Owen K.
dc.creatorSanz, Libia
dc.creatorBeutler, Markus
dc.creatorWright, Christine E.
dc.creatorCalvete Chornet, Juan José
dc.creatorWilliams, David J.
dc.creatorGutiérrez, José María
dc.date.accessioned2017-06-16T21:35:25Z
dc.date.available2017-06-16T21:35:25Z
dc.date.issued2014-10-14
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S1874391914003194es_ES
dc.identifier.issn1874-3919
dc.identifier.urihttp://hdl.handle.net/10669/30128
dc.description.abstractThere is no specific antivenom for the treatment of envenoming by the small-eyed snake, Micropechis ikaheka, a dangerous fossorial species endemic to Papua New Guinea, Irian Jaya (West Papua) and neighbouring islands. This study evaluated one marine (sea snake) and four terrestrial (tiger snake, brown snake, black snake and polyvalent) antivenoms, manufactured in Australia by bioCSL Limited, for their ability to immunoreact (‘antivenomic’ analysis) and neutralize enzymatic and toxic activities of M. ikaheka venom. All antivenoms neutralized lethality of the venom and attenuated, dose-dependently, myotoxic activity. The polyvalent antivenom also neutralized cardiotoxic activity. In contrast, antivenoms were ineffective in the neutralization of phospholipase A2 (PLA2) and anticoagulant activities. Antivenomics outcomes were in concordance with neutralization tests, for chromatographic peaks corresponding to α-neurotoxins of the three finger family, responsible for lethality, were quantitatively retained in the immunoaffinity columns, whereas peaks corresponding to PLA2s were immunocaptured only to a partial extent. The ability of antivenoms to neutralize lethal, i.e. neurotoxic, and myotoxic activities of M. ikaheka venom, which represent the most relevant clinical manifestations of envenoming, suggests that these antivenoms may provide paraspecific protection in humans, although the poor neutralization of PLA2 supports the need for well-designed clinical studies to not only determine which antivenoms are most appropriate for treatment of M. ikaheka envenoming, but to also fully describe the syndrome of envenoming caused by this beautiful, but lethal species. Biological significance Snakebite by the small-eyed snake, Micropechis ikaheka, in Papua New Guinea can be life-threatening. The predominant clinical features in this envenoming are neurotoxicity and systemic myotoxicity. Although it accounts for only a small proportion of snakebites on the mainland, 40% of snakebites on Karkar Island are attributed to bites by the Ikaheka snake. However, no specific antivenom is available for the treatment of M. ikaheka envenoming in Papua New Guinea. This study evaluated a panel of Australian bioCSL antivenoms for their paraspecific immunoreaction and neutralization of the toxic activities of M. ikaheka venom. All antivenoms exhibited strong immunorecognition of α-neurotoxins of the 3FTx family and neutralized the lethal, i.e. neurotoxic, and myotoxic activities of M. ikaheka venom. However, these antivenoms exhibited poor neutralization of PLA2 and anticoagulant activities. This study suggests that the Australian antivenoms may provide paraspecific protection against M. ikaheka venom in humans, a hypothesis that demands studies aimed at assessing whether these antivenoms neutralize neurotoxicity and myotoxicity in the clinical setting.es_ES
dc.description.sponsorshipMinisterio de Ciencia e Innovación/[BFU2010-17373]//Españaes_ES
dc.description.sponsorshipGeneralitat Valenciana/[PROMETEO/2010/005]//Españaes_ES
dc.description.sponsorshipPrograma Iberoamericano de Ciencia y Tecnología para el Desarrollo/[BIOTOX P211RT0412]/CYTED/Españaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B2-652]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceJournal of Proteomics; Volumen 110. 2014es_ES
dc.subjectMicropechis ikahekaes_ES
dc.subjectAntivenomses_ES
dc.subjectNeutralizationes_ES
dc.subjectAntivenomicses_ES
dc.subjectLethalityes_ES
dc.subjectMyotoxicityes_ES
dc.titlePreclinical efficacy of Australian antivenoms against the venom of the small-eyed snake, Micropechis ikaheka, from Papua New Guinea: An antivenomics and neutralization studyes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.jprot.2014.06.016
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-B2-652
dc.identifier.pmid24980637


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