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dc.creatorVargas Arroyo, Mariángela
dc.creatorSegura Ruiz, Álvaro
dc.creatorVillalta Arrieta, Mauren
dc.creatorHerrera Vega, María
dc.creatorGutiérrez, José María
dc.creatorLeón Montero, Guillermo
dc.date.accessioned2017-07-07T17:21:11Z
dc.date.available2017-07-07T17:21:11Z
dc.date.issued2015-01
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S1045105614000992es_ES
dc.identifier.issn1045-1056
dc.identifier.urihttps://hdl.handle.net/10669/30340
dc.description.abstractThere is a need to introduce innovations in the manufacture of snake antivenoms to increase the supply of these products worldwide. In this work, the fractionation of equine hyperimmune plasma with a new methodology that includes an aqueous two phase system (ATPS) as a primary purification step was compared with the traditional method of caprylic acid precipitation. Hyperimmune plasma from horses immunized with the venoms of three snakes from sub-Saharan Africa was used as starting material for the production of both formulations. After being adjusted to the same lethal neutralizing activity, both antivenoms were compared in terms of their immunoreactivity, neutralization of in vitro venom activities, physicochemical characteristics, and stability. Their performance in terms of yield and purity was also assessed. The neutralization profile of in vitro enzymatic activities and the immunoreactivity, analyzed by ELISA and antivenomic approaches, were very similar for both preparations. Likewise, they behaved similarly in stability studies. However, ATPS-fractionated antivenom showed improved physicochemical profile and immunochemical purity and yield, mainly owing to its lower protein content. Additionally, this methodology allowed the recovery of albumin as a byproduct. ATPS purification constitutes a promising technology for antivenom production and should be further evaluated at preclinical and clinical levels.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B2-090]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceBiologicals; Volumen 43, Número 1. 2015es_ES
dc.subjectSnake antivenomes_ES
dc.subjectAqueous two phase systemes_ES
dc.subjectCaprylic acides_ES
dc.subjectImmunoglobulinses_ES
dc.subjectSnake venomes_ES
dc.titlePurification of equine whole IgG snake antivenom by using an aqueous two phase system as a primary purification stepes_ES
dc.typeartículo científicoes_ES
dc.identifier.doi10.1016/j.biologicals.2014.10.003
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-B2-090
dc.identifier.pmid25458474


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