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dc.creatorChacón Valverde, Francisco
dc.creatorOviedo Cerdas, Andrea
dc.creatorEscalante Muñoz, Teresa
dc.creatorSolano Trejos, María Gabriela
dc.creatorRucavado Romero, Alexandra
dc.creatorGutiérrez, José María
dc.date.accessioned2017-07-07T20:09:23Z
dc.date.available2017-07-07T20:09:23Z
dc.date.issued2015-01
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0041010114005844es_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/30343
dc.description.abstractThe potency of antivenoms is assessed by analyzing the neutralization of venom-induced lethality, and is expressed as the Median Effective Dose (ED50). The present study was designed to investigate the pathophysiological mechanisms responsible for lethality induced by the venom of Bothrops asper, in the experimental conditions used for the evaluation of the neutralizing potency of antivenoms. Mice injected with 4 LD50s of venom by the intraperitoneal route died within ∼25 min with drastic alterations in the abdominal organs, characterized by hemorrhage, increment in plasma extravasation, and hemoconcentration, thus leading to hypovolemia and cardiovascular collapse. Snake venom metalloproteinases (SVMPs) play a predominat role in lethality, as judged by partial inhibition by the chelating agent CaNa2EDTA. When venom was mixed with antivenom, there was a venom/antivenom ratio at which hemorrhage was significantly reduced, but mice died at later time intervals with evident hemoconcentration, indicating that other components in addition to SVMPs also contribute to plasma extravasation and lethality. Pretreatment with the analgesic tramadol did not affect the outcome of the neutralization test, thus suggesting that prophylactic (precautionary) analgesia can be introduced in this assay. Neutralization of lethality in mice correlated with neutralization of in vitro coagulant activity in human plasma.es_ES
dc.description.sponsorshipConsejo Nacional de Rectores/[741-B2-652]/FEES-CONARE/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B2-652]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 93. 2015es_ES
dc.subjectAntivenomes_ES
dc.subjectBothrops asperes_ES
dc.subjectLethalityes_ES
dc.subjectNeutralizationes_ES
dc.subjectHemorrhagees_ES
dc.subjectHemoconcentratones_ES
dc.titleThe lethality test used for estimating the potency of antivenoms against Bothrops asper snake venom: Pathophysiological mechanisms, prophylactic analgesia, and a surrogate in vitro assayes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.toxicon.2014.11.223
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-B2-652
dc.identifier.pmid25447772


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