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dc.creatorNavarro Arias, Diego
dc.creatorVargas Arroyo, Mariángela
dc.creatorHerrera Vega, María
dc.creatorSegura Ruiz, Álvaro
dc.creatorGómez Argüello, Aarón
dc.creatorVillalta Arrieta, Mauren
dc.creatorRamírez Arguedas, Nils Antonio
dc.creatorWilliams, David J.
dc.creatorGutiérrez, José María
dc.creatorLeón Montero, Guillermo
dc.date.accessioned2017-07-18T18:59:28Z
dc.date.available2017-07-18T18:59:28Z
dc.date.issued2016-09-15
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0041010116301726es_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/30372
dc.description.abstractA chicken-derived antivenom (ChDAv) towards taipan snake (Oxyuranus scutellatus) venom was produced by purifying anti-taipan IgY from egg yolks of hens immunized with taipan venom. The productivity, antivenomic profile, neutralization ability, pharmacokinetic properties and immunogenicity of the ChDAv were compared with those of an antivenom produced in horses (EDAv). We found that 382 eggs are required to produce the mass of anti-taipan antibodies contained in one liter of equine hyperimmune plasma, and that 63 chickens would be needed to generate the amount of anti-taipan antibodies annually produced by one horse. It was estimated that, in Costa Rica, the production of antitaipan antibodies could be 40% cheaper if chickens were used as immunoglobulin source, instead of horses. During antivenomic assessment, ChDAv showed lower ability to immunocapture the a subunit of taipoxin, the most important neurotoxin in the venom. ChDAv showed a lower ability to neutralize the coagulant and lethal activities of taipan venom. ChDAv was more immunogenic in rabbits than EDAv, probably due to the fact that chickens are phylogenetically more distant to rabbits than horses. This finding may explain why clearance from rabbit bloodstream was faster for chicken-IgY than for equineIgG in a pharmacokinetic study. In conclusion, the production of anti-taipan antivenom was less effective when chicken egg yolks were used as source of immunoglobulins instead of horses.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B3-017]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 120. 2016es_ES
dc.subjectOxyuranus scutellatuses_ES
dc.subjectSnake venomes_ES
dc.subjectAntivenomes_ES
dc.subjectForeignnesses_ES
dc.subjectPharmacokineticses_ES
dc.subjectAntibody responsees_ES
dc.titleDevelopment of a chicken-derived antivenom against the taipan snake (Oxyuranus scutellatus) venom and comparison with an equine antivenomes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.toxicon.2016.06.018
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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