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dc.creatorAtanasov, Kostadin E.
dc.creatorBarboza Barquero, Luis Orlando
dc.creatorTiburcio, Antonio F.
dc.creatorAlcázar, Rubén
dc.date.accessioned2017-08-25T18:52:19Z
dc.date.available2017-08-25T18:52:19Z
dc.date.issued2016
dc.identifier.citationhttp://journal.frontiersin.org/article/10.3389/fpls.2016.00401/fulles_ES
dc.identifier.urihttp://hdl.handle.net/10669/73029
dc.description.abstractGuazatine is a potent inhibitor of polyamine oxidase (PAO) activity. In agriculture, guazatine is used as non-systemic contact fungicide efficient in the protection of cereals and citrus fruits against disease. The composition of guazatine is complex, mainly constituted by a mixture of synthetic guanidated polyamines (polyaminoguanidines). Here, we have studied the effects from exposure to guazatine in the weed Arabidopsis thaliana. We report that micromolar concentrations of guazatine are sufficient to inhibit growth of Arabidopsis seedlings and induce chlorosis, whereas germination is barely affected. We observed the occurrence of quantitative variation in the response to guazatine between 107 randomly chosen Arabidopsis accessions. This enabled us to undertake genome-wide association (GWA) mapping that identified a locus on chromosome one associated with guazatine tolerance. CHLOROPHYLLASE 1 (CLH1) within this locus was studied as candidate gene, together with its paralog (CLH2). The analysis of independent clh1-2, clh1-3, clh2-3, clh2-2, and double clh1-2 clh2-3 mutant alleles indicated that CLH1 and/or CLH2 loss-of-function or expression down-regulation promote guazatine tolerance in Arabidopsis. We report a natural mechanism by which Arabidopsis populations can overcome toxicity by the fungicide guazatine.es_ES
dc.description.sponsorshipMinisterio de Ciencia e Innovación/[RYC-2011-07847]//Españaes_ES
dc.description.sponsorshipMinisterio de Economía y Competitividad/[BFU2013-41337-P]//Españaes_ES
dc.description.sponsorshipFondo Europeo de Desarrollo Regional/[BFU2013-41337-P]/FEDER/Españaes_ES
dc.description.sponsorshipMarie Curie Career Integration Grants/[PCIG10-GA-2011-303568]/CIG/Españaes_ES
dc.description.sponsorshipMinisterio de Ciencia e Innovación/[BIO2011-29683]//Españaes_ES
dc.language.isoen_USes_ES
dc.sourceFrontiers in Plant Science; Volume 7, Article 401. 2016es_ES
dc.subjectGuazatinees_ES
dc.subjectNatural variationes_ES
dc.subjectPolyaminoguanidineses_ES
dc.subjectPopulation geneticses_ES
dc.titleGenome wide association mapping for the tolerance to the polyamine oxidase inhibitor guazatine in arabidopsis thalianaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.3389/fpls.2016.00401
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Centro para Investigaciones en Granos y Semillas (CIGRAS)es_ES


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