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dc.creatorLomonte, Bruno
dc.creatorTarkowski, Andrej
dc.creatorBagge, Ulf
dc.creatorHanson, Lars Ake
dc.date.accessioned2017-09-13T19:31:37Z
dc.date.available2017-09-13T19:31:37Z
dc.date.issued1994
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/0006295294905258es_ES
dc.identifier.issn0006-2952
dc.identifier.urihttp://hdl.handle.net/10669/73146
dc.description.abstractBasic phospholipases A2 from the venom of Bothrops asper exhibit skeletal muscle damaging activity in vivo, and cytolytic activity to a variety of cell types in culture. Glycosaminoglycans of the heparin/heparan sulfate family were found to be potent blockers of the cytolytic action in vitro, and, as well, to be able to neutralize the muscle damaging activity of purified myotoxins and crude venom in vivo. However, the neutralizing effect of heparins was more potent in vitro than in vivo. The cytolytic activity of myotoxin II (a lysine-49 phospholipase A2 isoform) and its inhibition by heparin was characterized. The neutralizing effect of heparin did not depend on its anticoagulant activity, since both standard heparin and heparin with low affinity for antithrombin (LA-heparin) had a similar efficiency. Heparan sulfate and low molecular mass heparin (5 kDa) also neutralized myotoxin II. In contrast, different heparin-derived disaccharides were unable to block cytolysis, implying a requirement for a longer carbohydrate chain structure for the interaction with the protein. By affinity chromatography and gel diffusion, it was demonstrated that heparins form a complex with all isoforms of basic venom myotoxins, held at least in part by electrostatic interactions. The phospholipase A2 activity of myotoxin III, a related aspartate-49 isoform from the same venom, was unaffected by heparins, despite the fact that its myotoxic activity was inhibited, indicating a dissociation of the two actions.es_ES
dc.description.sponsorshipThe Swedish Agency for Research Cooperation with Developing Countries//SAREC/Sueciaes_ES
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Tecnologías de Costa Rica/[]/CONICIT/Costa Ricaes_ES
dc.description.sponsorshipInternational Foundation for Science/[F/1388-2]//Sueciaes_ES
dc.description.sponsorshipSwedish Medical Research Council/[00663]//Sueciaes_ES
dc.language.isoen_USes_ES
dc.sourceBiochemical Pharmacology; Volumen 47, Número 9. 1994es_ES
dc.subjectVenomes_ES
dc.subjectMyotoxines_ES
dc.subjectSnakees_ES
dc.subjectHeparines_ES
dc.subjectPhospholipase A2es_ES
dc.subjectCytotoxicityes_ES
dc.subjectSnake venomes_ES
dc.titleNeutralization of the cytolytic and myotoxic activities of phospholipases A2 from Bothrops asper snake venom by glycosaminoglycans of the heparin/heparan sulfate familyes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doi10.1016/0006-2952(94)90525-8
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.pmid8185661


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