Show simple item record

dc.creatorMonge Acuña, Andrea A.
dc.creatorFornaguera Trías, Jaime
dc.date.accessioned2017-12-18T13:34:45Z
dc.date.available2017-12-18T13:34:45Z
dc.date.issued2009
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0165027009003501?via%3Dihubes_ES
dc.identifier.issn0165-0270
dc.identifier.urihttp://hdl.handle.net/10669/73691
dc.description.abstractDetermination of γ-aminobutyric acid (GABA), glutamate (Glu) and glutamine (Gln) in animal models has been important to understand the normal function and clinical aspects of some neurological diseases. Quantification of these amino acid transmitters has conventionally been performed by using a high performance liquid chromatography (HPLC) system. This paper describes an improved HPLC method with electrochemical detection for glutamate, glutamine and GABA determination in brain homogenates. The protocol is based on a precolumn derivatization of amino acids with o-phthalaldehyde and sodium sulfite, a separation through a C18, 5 μm particle size column and an isocratic elution. Several modifications of previous works on methanol percentage, pH, temperature, flow rate and derivatization solution concentration were done to obtain a suitable protocol for amino acid quantification in brain homogenate samples. Total elution time is 35 min approximately. Technical requirements and laboratory expenses of this new protocol are minimal. This technique showed high linearity, repeatability and accuracy.es_ES
dc.description.sponsorshipVicerrectoria de Investigación, Universidad de Costa Rica/[422-A5 524]/VINV/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceJournal of Neuroscience Method, Vol. 183 (2), pp. 176–181es_ES
dc.subjectGABAes_ES
dc.subjectGlutamatees_ES
dc.subjectGlutaminees_ES
dc.subjectHPLCes_ES
dc.subjectIsocratic elutiones_ES
dc.subjectNeurotransmitterses_ES
dc.subjectHippocampuses_ES
dc.subjectRatses_ES
dc.titleA high performance liquid chromatography method with electrochemical detection of gamma-aminobutyric acid, glutamate and glutamine in rat brain homogenateses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.jneumeth.2009.06.042
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Medicina::Escuela de Medicinaes_ES
dc.identifier.pmid19596377


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record