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dc.creatorAngulo Ugalde, Yamileth
dc.creatorLomonte, Bruno
dc.date.accessioned2018-02-27T20:26:11Z
dc.date.available2018-02-27T20:26:11Z
dc.date.issued2005
dc.identifier.citationhttp://onlinelibrary.wiley.com/doi/10.1002/cbf.1208/abstractes_ES
dc.identifier.issn1099-0844
dc.identifier.urihttp://hdl.handle.net/10669/74165
dc.description.abstractGroup II phospholipase A2 (PLA2) myotoxins isolated from Viperidae/Crotalidae snake venoms induce a rapid cytolytic effect upon diverse cell types in vitro. Previous studies suggested that this effect could be more pronounced on skeletal muscle myotubes than on other cell types, including undifferentiated myoblasts. This study utilized the murine skeletal muscle C2C12 cell line to investigate whether differentiated myotubes are more susceptible than myoblasts, and if this characteristic is specific for the group II myotoxic PLA2s. The release of lactic dehydrogenase was quantified as a measure of cytolysis, 3 h after cell exposure to different group II PLA2s purified from Bothrops asper, Atropoides nummifer, Cerrophidion godmani, and Bothriechis schlegelii venoms. In addition, susceptibility to lysis induced by synthetic melittin and group III PLA2 from bee (Apis mellifera) venom, as well as by anionic, cationic, and neutral detergents, was comparatively evaluated on the two cultures. Myotubes were significantly more susceptible to group II PLA2 myotoxins, but not to the other agents tested, under the same conditions. Moreover, the increased susceptibility of myotubes over myoblasts was also demonstrated with two cytolytic synthetic peptides, derived from the C-terminal region of Lys49 PLA2 myotoxins, that reproduce the action of their parent proteins. These results indicate that fusion and differentiation of myoblasts into myotubes induce changes that render these cells more susceptible to the toxic mechanism of group II PLA2 myotoxins, but not to general perturbations of membrane homeostasis. Such changes are likely to involve myotoxin acceptor site(s), which remain(s) to be identified.es_ES
dc.description.sponsorshipInternational Foundation for Science/[F/2766-2]/IFS/Sueciaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-99-269]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A3-513]/UCR/Costa Ricaes_ES
dc.description.sponsorshipConsejo Nacional para Investigaciones Científicas y Tecnológicas/[FV058-02]/CONICIT-FORINVES/Costa Ricaes_ES
dc.description.sponsorshipNeTropica Sweden-Central America network/[]//Sueciaes_ES
dc.language.isoen_USes_ES
dc.sourceCell Biochemistry and Function 23, 307-313 (2005)es_ES
dc.subjectmyoblastes_ES
dc.subjectmyotubees_ES
dc.subjectskeletal musclees_ES
dc.subjectPhospholipase A2es_ES
dc.subjectmyotoxines_ES
dc.subjectSnake venomes_ES
dc.titleDifferential susceptibility of C2C12 myoblasts and myotubes to group II phospholipase A2 myotoxins from crotalid snake venomses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1002/cbf.1208
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Medicina::Escuela de Medicinaes_ES
dc.identifier.codproyecto741-99-269
dc.identifier.codproyecto741-A3-513
dc.identifier.pmid15657942


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