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dc.creatorNavarro Hoyos, Mirtha
dc.creatorMoreira González, Ileana
dc.creatorArnáez Serrano, Elizabeth
dc.creatorQuesada Mora, Silvia
dc.creatorAzofeifa Cordero, Gabriela
dc.creatorAlvarado Corella, Luis Diego
dc.creatorMonagas, Maria J.
dc.date.accessioned2018-05-15T20:27:30Z
dc.date.available2018-05-15T20:27:30Z
dc.date.issued2017-10-19
dc.identifier.citationhttp://www.mdpi.com/2223-7747/6/4/50es_ES
dc.identifier.issn2223-7747
dc.identifier.urihttp://hdl.handle.net/10669/74616
dc.description.abstractThe phenolic composition of aerial parts from Petiveria alliaceae L., Phyllanthus niruri L. and Senna reticulata Willd., species commonly used in Costa Rica as traditional medicines, was studied using UPLC-ESI-TQ-MS on enriched-phenolic extracts. Comparatively, higher values of total phenolic content (TPC), as measured by the Folin-Ciocalteau method, were observed for P. niruri extracts (328.8 gallic acid equivalents/g) than for S. reticulata (79.30 gallic acid equivalents/g) whereas P. alliaceae extract showed the lowest value (13.45 gallic acid equivalents/g). A total of 20 phenolic acids and proanthocyanidins were identified in the extracts, including hydroxybenzoic acids (benzoic, 4-hydroxybenzoic, gallic, prochatechuic, salicylic, syringic and vanillic acids); hydroxycinnamic acids (caffeic, ferulic, and p-coumaric acids); and flavan-3-ols monomers [(+)-catechin and (−)-epicatechin)]. Regarding proanthocyanidin oligomers, five procyanidin dimers (B1, B2, B3, B4, and B5) and one trimer (T2) are reported for the first time in P. niruri, as well as two propelargonidin dimers in S. reticulata. Additionally, P. niruri showed the highest antioxidant DPPH and ORAC values (IC50 of 6.4 μg/mL and 6.5 mmol TE/g respectively), followed by S. reticulata (IC50 of 72.9 μg/mL and 2.68 mmol TE/g respectively) and P. alliaceae extract (IC50 >1000 μg/mL and 1.32 mmol TE/g respectively). Finally, cytotoxicity and selectivity on gastric AGS and colon SW20 adenocarcinoma cell lines were evaluated and the best values were also found for P. niruri (SI = 2.8), followed by S. reticulata (SI = 2.5). Therefore, these results suggest that extracts containing higher proanthocyanidin content also show higher bioactivities. Significant positive correlation was found between TPC and ORAC (R2 = 0.996) as well as between phenolic content as measured by UPLC-DAD and ORAC (R2 = 0.990). These findings show evidence for the first time of the diversity of phenolic acids in P. alliaceae and S. reticulata, and the presence of proanthocyanidins as minor components in latter species. Of particular relevance is the occurrence of proanthocyanidin oligomers in phenolic extracts from P. niruri and their potential bioactivityes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[115-B0-653]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourcePlants. Vol 6. Núm 4. pp 1-13es_ES
dc.subjectP. alliaceaees_ES
dc.subjectP. niruries_ES
dc.subjectS. reticulataes_ES
dc.subjectUPLCes_ES
dc.subjectTQ-ESI-MSes_ES
dc.subjectProanthocyanidinses_ES
dc.subjectMass spectrometryes_ES
dc.subjectAntioxidantes_ES
dc.subjectCytotoxicityes_ES
dc.titleProanthocyanidin Characterization, Antioxidant and Cytotoxic Activities of Three Plants Commonly Used in Traditional Medicine in Costa Rica: Petiveria alliaceae L., Phyllanthus niruri L. and Senna reticulataWilldes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.3390/plants6040050
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigaciones en Productos Naturales (CIPRONA)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Químicaes_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Medicina::Escuela de Medicinaes_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biologíaes_ES
dc.identifier.codproyecto115-B0-653


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