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dc.creatorHeringer, Angelo Schuabb
dc.creatorSteinmacher, Douglas A.
dc.creatorFraga, Hugo Pacheco de Freitas
dc.creatorVieira, Leila do Nascimento
dc.creatorMontagna, Tiago
dc.creatorQuinga, Liliana Alexandra Pila
dc.creatorQuoirin, Marguerite Germaine Ghislaine
dc.creatorJiménez García, Víctor
dc.creatorGuerra, Miguel Pedro
dc.date.accessioned2018-08-31T16:51:09Z
dc.date.available2018-08-31T16:51:09Z
dc.date.issued2014-11
dc.identifier.citationhttps://www.sciencedirect.com/science/article/pii/S0304423814005287es_ES
dc.identifier.issn0304-4238
dc.identifier.urihttp://hdl.handle.net/10669/75562
dc.description.abstractBactris gasipaes Kunth (Arecaceae) is an Amazonian palm cultivated mainly for the production of fruits and heart of palm. Biotechnological tools based on somatic embryogenesis (SE) can be used for improvement and germplasm conservation of this species. The efficiency of different culture systems and culture medium composition were evaluated to optimize the efficiency of SE. The study also evaluated global DNA methylation levels, total protein and starch contents, and alcohol dehydrogenase (ADH) activity. The RITA® bioreactor showed enhanced multiplication of somatic embryos with increased protein, starch levels and ADH activity. Furthermore, a low DNA global methylation rate (27.52%) was observed, suggesting its relationship with the expression of proteins associated with the maturation of somatic embryos and their subsequent conversion to plantlets. Somatic embryo maturation was successfully achieved on Petri dishes and abscisic acid (ABA) supplementation in the culture medium. Chlorophyll pigmented somatic embryos were obtained in Petri dishes containing PGR-free culture medium, and RITA® provided the greatest number of plantlets to be acclimatized after reaching 6-7 cm in length. In the acclimatization step, the best results were obtained with plantlets derived from RITA® after reaching 6-7 cm in length. These new findings shed light on the importance of defining adequate culture systems for different steps of in vitro regenerative protocols and represent a breakthrough in the in vitro morphogenesis process of peach palm, resulting in a large number of somatic plantlets with enhanced physiological and morphological features.es_ES
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico/CNPq/[478393/2013-0]/CNPq/Brasiles_ES
dc.description.sponsorshipFundação de Amparo a Pesquisa e Inovação do Estado de Santa Catarina/[3770/2012]/FAPESC/Brasiles_ES
dc.description.sponsorshipFundação de Amparo a Pesquisa e Inovação do Estado de Santa Catarina/[2780/2012-4]/FAPESC/Brasiles_ES
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas/[490552/2010-2]/CONICIT/es_ES
dc.language.isoen_USes_ES
dc.relation.ispartof
dc.sourceScientia Horticulturae, Vol.179, pp.284-292es_ES
dc.subjectSomatic embryogenesises_ES
dc.subjectTemporary immersion systemes_ES
dc.subjectPejibayees_ES
dc.subjectAbscisic acides_ES
dc.subjectDNA methylationes_ES
dc.subject631.523 3 Genética agrícolaes_ES
dc.titleImproved high-efficiency protocol for somatic embryogenesis in Peach Palm (Bactris gasipaes Kunth) using RITA® temporary immersion systemes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.date.updated2018-08-09T04:06:27Z
dc.identifier.doi10.1016/j.scienta.2014.09.041
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Centro para Investigaciones en Granos y Semillas (CIGRAS)es_ES


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