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dc.creatorVicente Santos, Amanda
dc.creatorMoreira Soto, Andrés
dc.creatorSoto Garita, Claudio
dc.creatorChaverri Sánchez, Luis Guillermo
dc.creatorChaves Ramírez, Andrea
dc.creatorDrexler, Jan Felix
dc.creatorMorales Acuña, Juan Alberto
dc.creatorAlfaro Alarcón, Alejandro
dc.creatorRodríguez Herrera, Bernal
dc.creatorCorrales Aguilar, Eugenia
dc.date.accessioned2019-02-25T21:23:11Z
dc.date.available2019-02-25T21:23:11Z
dc.date.issued2017-05-18
dc.identifier.citationhttps://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0005537es_ES
dc.identifier.issn1935-2735
dc.identifier.urihttps://hdl.handle.net/10669/76611
dc.description.abstractSeveral studies have shown Dengue Virus (DENV) nucleic acids and/or antibodies present in Neotropical wildlife including bats, suggesting that some bat species may be susceptible to DENV infection. Here we aim to elucidate the role of house-roosting bats in the DENV transmission cycle. Bats were sampled in households located in high and low dengue incidence regions during rainy and dry seasons in Costa Rica. We captured 318 bats from 12 different species in 29 households. Necropsies were performed in 205 bats to analyze virus presence in heart, lung, spleen, liver, intestine, kidney, and brain tissue. Histopathology studies from all organs showed no significant findings of disease or infection. Sera were analyzed by PRNT90 for a seroprevalence of 21.2% (51/241), and by PCR for 8.8% (28/318) positive bats for DENV RNA. From these 28 bats, 11 intestine samples were analyzed by RT-PCR. Two intestines were DENV RNA positive for the same dengue serotype detected in blood. Viral isolation from all positive organs or blood was unsuccessful. Additionally, viral load analyses in positive blood samples by qRT-PCR showed virus concentrations under the minimal dose required for mosquito infection. Simultaneously, 651 mosquitoes were collected using EVS-CO2 traps and analyzed for DENV and feeding preferences (bat cytochrome b). Only three mosquitoes were found DENV positive and none was positive for bat cytochrome b. Our results suggest an accidental presence of DENV in bats probably caused from oral ingestion of infected mosquitoes. Phylogenetic analyses suggest also a spillover event from humans to bats. Therefore, we conclude that bats in these urban environments do not sustain DENV amplification, they do not have a role as reservoirs, but function as epidemiological dead end hosts for this virus.es_ES
dc.language.isoen_USes_ES
dc.relation.ispartof
dc.sourcePlos Neglected Tropical Diseases, vol. 11(5), art. e0005537es_ES
dc.subjectDengue Viruses_ES
dc.subjectBatses_ES
dc.subjectCosta Ricaes_ES
dc.subjectDead end hostses_ES
dc.subject599.4 Chiroptera (Quirópteros, Murciélagos)es_ES
dc.titleNeotropical bats that co-habit with humans function as dead-end hosts for dengue viruses_ES
dc.typeartículo científicoes_ES
dc.date.updated2019-01-11T21:54:04Z
dc.identifier.doi10.1371/journal.pntd.0005537
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiologíaes_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biologíaes_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET)es_ES
dc.identifier.codproyecto803-B4-656


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