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dc.creatorVenegas Solís, Francisco A.
dc.creatorKöllisch, Gabriele
dc.creatorMark, Kerstin
dc.creatorDiederich, Wibke E.
dc.creatorKaufmann, Andreas
dc.creatorBauer, Stefan
dc.creatorChavarría Vargas, Max
dc.creatorAraya Barrantes, Juan José
dc.creatorGarcía Piñeres, Alfonso J.
dc.date.accessioned2020-01-15T21:09:50Z
dc.date.available2020-01-15T21:09:50Z
dc.date.issued2019
dc.identifier.citationhttps://www.nature.com/articles/s41598-019-50038-xes_ES
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10669/80295
dc.description.abstractViolacein, an indole-derived, purple-colored natural pigment isolated from Chromobacterium violaceum has shown multiple biological activities. In this work, we studied the effect of violacein in different immune cell lines, namely THP-1, MonoMac 6, ANA-1, Raw 264.7 cells, as well as in human peripheral blood mononuclear cells (PBMCs). A stimulation of TNF-α production was observed in murine macrophages (ANA-1 and Raw 264.7), and in PBMCs, IL-6 and IL-1β secretion was detected. We obtained evidence of the molecular mechanism of activation by determining the mRNA expression pattern upon treatment with violacein in Raw 264.7 cells. Incubation with violacein caused activation of pathways related with an immune and inflammatory response. Our data utilizing TLR-transfected HEK-293 cells indicate that violacein activates the human TLR8 (hTLR8) receptor signaling pathway and not human TLR7 (hTLR7). Furthermore, we found that the immunostimulatory effect of violacein in PBMCs could be suppressed by the specific hTLR8 antagonist, CU-CPT9a. Finally, we studied the interaction of hTLR8 with violacein in silico and obtained evidence that violacein could bind to hTLR8 in a similar fashion to imidazoquinoline compounds. Therefore, our results indicate that violacein may have some potential in contributing to future immune therapy strategies.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[801-B2-519]/UCR/Costa Ricaes_ES
dc.description.sponsorshipMinisterio de Ciencia, Tecnología y Telecomunicaciones/[FI-497-11]/MICITT/Costa Ricaes_ES
dc.description.sponsorshipMinisterio de Ciencia, Tecnología y Telecomunicaciones/[DFG-TR84]/MICITT/Costa Ricaes_ES
dc.description.sponsorshipMinisterio de Ciencia, Tecnología y Telecomunicaciones/[DFG-KFO325]/MICITT/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.relation.ispartof
dc.sourceScientific Reports, vol.9(13661), pp.1-17es_ES
dc.subjectBacteriales_ES
dc.subjectViolaceines_ES
dc.subjectImmunostimulatoryes_ES
dc.titleThe Bacterial Product Violacein Exerts an Immunostimulatory Effect Via TLR8es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.date.updated2019-11-26T21:35:47Z
dc.identifier.doi10.1038/s41598-019-50038-x
dc.description.procedenceUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Químicaes_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigaciones en Productos Naturales (CIPRONA)es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM)es_ES
dc.identifier.codproyecto801-B2-519


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