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dc.creatorNeri Castro, Edgar
dc.creatorLomonte, Bruno
dc.creatorValdés, Mariel
dc.creatorPonce López, Roberto
dc.creatorBénard Valle, Melisa
dc.creatorBorja, Miguel
dc.creatorStrickland, Jason L.
dc.creatorJones, Jason M.
dc.creatorGrünwald, Christoph
dc.creatorZamudio, Fernando
dc.creatorAlagón Cano, Alejandro
dc.date.accessioned2020-01-22T20:34:00Z
dc.date.available2020-01-22T20:34:00Z
dc.date.issued2019
dc.identifier.citationhttps://www.sciencedirect.com/science/article/pii/S1874391918303439?via%3Dihubes_ES
dc.identifier.issn1874-3919
dc.identifier.urihttp://hdl.handle.net/10669/80364
dc.description.abstractVenoms of the three species of Ophryacus (O. sphenophrys, O. smaragdinus, and O. undulatus), a viperid genus endemic to Mexico, were analyzed for the first time in the present work. The three venoms lacked procoagulant activity on human plasma, but induced hemorrhage and were highly lethal to mice. These venoms also displayed proteolytic and phospholipase A2 activities in vitro. The venom of O. sphenophrys was the most lethal and caused hind-limb paralysis in mice. Proteomic profiling of O. sphenophrys venom showed a predominance of metalloproteinase (34.9%), phospholipase A2 (24.8%) and serine protease (17.1%) in its composition. Strikingly, within its PLA2 components, 12.9% corresponded to a Crotoxin-like heterodimer, here named Sphenotoxin, which was not found in the other two species of Ophryacus. Sphenotoxin, like Crotoxin, is composed of non-covalently bound A and B subunits. Partial amino acid sequence was obtained for Sphenotoxin B and was similar (78–89%) to other subunits described. The mouse i.v. LD50 of Sphenotoxin at 1:1M radio was 0.16 μg/g. Also, like Crotoxin, Sphenotoxin induced a potent neuromuscular blockade in the phrenic nerve-diaphragm preparation. Ophryacus is the fifth genus and O. sphenophrys the third non-rattlesnake species shown to contain a novel Crotoxin-like heterodimeric β-neurotoxin. Biological significance: Ophryacus is an endemic genus of semi-arboreal pitvipers from Mexico that includes three species with restricted distributions. Little is known about the natural history of these species and nothing is known about the properties of their venoms. Research on these species' venoms could generate relevant information regarding venom composition of Mexican pitvipers. Additionally, research into the presence of neurotoxic Crotoxin-like molecules outside of rattlesnakes (genera Crotalus and Sistrurus) has identified this molecule in several new genera. Knowing which genera and species possess neurotoxic components is important to fully understand the repercussions of snakebites, the interaction with prey and predators, and the origin, evolution, and phylogenetic distribution of Crotoxin-like molecules during the evolutionary history of pitvipers. Our study expands current knowledge regarding venom's compositions and function from Mexican pitvipers, providing a comparative venom characterization of major activities in the three Ophryacus species. Additionally, the discovery and characterization of a novel Crotoxin-like molecule, here named Sphenotoxin, in O. sphenophrys, and the detailed protein composition of O. sphenophrys venom supports the hypotheses that Crotoxin-like -β-neurotoxins are more widespread than initially thought.es_ES
dc.description.sponsorshipDirección General de Asuntos del Personal Académico/[DGAPA-PAPIIT: IN207218]/DGAPA/Méxicoes_ES
dc.description.sponsorshipConsejo Nacional de Ciencia y Tecnología/[703353]/CONACyT/Costa Ricaes_ES
dc.description.sponsorshipConsejo Nacional de Ciencia y Tecnología/[254145]/CONACyT/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceJournal of Proteomics, vol.192, pp.196-207es_ES
dc.subjectOphryacuses_ES
dc.subjectSnake venomes_ES
dc.subjectCrotoxines_ES
dc.titleVenom characterization of the three species of Ophryacus and proteomic profiling of O. sphenophrys unveils Sphenotoxin, a novel Crotoxin-like heterodimeric β-neurotoxines_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doi10.1016/j.jprot.2018.09.002
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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