Show simple item record

dc.creatorLomonte, Bruno
dc.creatorCamacho Umaña, Erika
dc.creatorFernández Ulate, Julián
dc.creatorSalas Arruz, María
dc.creatorZavaleta Martínez Vargas, Alfonso
dc.date.accessioned2020-01-28T15:50:09Z
dc.date.available2020-01-28T15:50:09Z
dc.date.issued2019
dc.identifier.citationhttps://www.sciencedirect.com/science/article/pii/S0041010119301989?via%3Dihubes_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/80378
dc.description.abstractVenoms from Micrurus (New World coral snakes) display potent peripheral neurotoxicity which may cause death by respiratory paralysis, yet many are poorly or not characterized. The major venom components of coral snakes are three-finger toxins (3FTxs) and phospholipases A2, whose proportions vary among species. As a trend, venoms of Micrurus from South America contain high proportions of 3FTxs, in contrast to most North and Central American species. Micrurus tschudii tschudii, the ‘Desert coral snake’ from Perú, displays an extreme 3FTx-predominant venom phenotype, with ∼95% of its proteome belonging to this protein family. This study evaluated the toxicity of its major 3FTxs in mice. A lethal 3FTx, here named tschuditoxin-I, was purified and sequenced by MALDI-TOF-TOF and N-terminal degradation. Tschuditoxin-I showed highest similarity to MS-1, a short-chain α- neurotoxin from the aquatic, fish-eating coral snake M. surinamensis. The single amino acid substitution between these two toxins maps at the tip of the first β-stranded ‘finger’ in the modeled structure of tschuditoxin-I, suggesting it may have a role in interaction with its target, which remains to be investigated. Owing to its lethal action, tschuditoxin-I is likely to be medically relevant in envenomings. In spite of its 74% sequence identity with an α-neurotoxin of M. nigrocinctus, an equine antivenom raised against venom of the latter did not immunorecognize tschuditoxin-I or M. t. tschudii venom by ELISA. This underscores the need of characterizing the biochemical and immunological properties of the main toxic components of Micrurus venoms, aiming to improve the limited para-specific coverage of current antivenoms.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B7-608]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon, vol.167, pp.144-151es_ES
dc.subjectMicruruses_ES
dc.subjectCoral snakees_ES
dc.subjectMicrurus t. tschudiies_ES
dc.subjectThree-finger toxinses_ES
dc.subjectsnake venomes_ES
dc.titleThree-finger toxins from the venom of Micrurus tschudii (desert coral snake): isolation and characterization of tschuditoxin-Ies_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doi10.1016/j.toxicon.2019.06.023
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-B7-608


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record