Novel three-finger toxins from Micrurus dumerilii and Micrurus mipartitus coral snake venoms: phylogenetic relationships and characterization of Clarkitoxin-I-Mdum
Rey Suárez, Paola
Saldarriaga Córdoba, Mónica María
Torres Lamus, Uday Daniel
Marin Villa, Marcel
Núñez Rangel, Vitelbina
MetadataShow full item record
Micrurus mipartitus and M. dumerilii are the most medically important coral snakes in Colombia. Proteomic characterization of their venoms has previously shown that proteins of the three-finger toxin (3FTx) family are abundant components, especially in M. mipartitus (61%) and to a lesser extent in M. dumerilii (28%). In order to increase knowledge on these toxins, in this work a major 3FTx of M. dumerilii venom (8% of the venom proteins), named Clarkitoxin-I-Mdum, was isolated and characterized. Its amino acid sequence comprises 66 residues, with an isotope-averaged molecular mass of 7537 ± 2 Da and a theoretical pI of 9.36, presenting the conserved pattern of eight cysteines that classifies it as a short-chain (type I) 3FTx. Clarkitoxin-I-Mdum was not lethal to mice by intravenous or intracerebroventricular route and was not cytolytic to myogenic cells in vitro. On the other hand, five coding sequences for 3FTxs were obtained from the venom gland of M. mipartitus. These novel toxin sequences were named Mm3FTx-01 to Mm3FTx-05, all of them also presenting the eight conserved cysteines of short-chain 3FTxs. Phylogenetic analysis revealed high variability of 3FTxs from Micrurus, and ELISA using antibodies raised to the major 3FTxs from M. mipartitus and M. dumerilii confirmed their immunochemical divergence. These results highlight the relevance of performing further studies aiming at a deeper understanding of the functional and antigenic relationships among specific Micrurus toxins, with important implications for the production of antivenoms.
- Microbiología 
Showing items related by title, author, creator and subject.
Consumo de un pez por parte de la serpiente coral Micrurus alleni(Serpentes: Elapidae) en Costa Rica Solórzano López, Alejandro
Incubación de los huevos y nacimiento en la coral gargantilla, Micrurus mipartitus hertwigi (Serpentes: Elapidae) en Costa Rica Solórzano López, Alejandro; Cerdas Fallas, Luis
Snake venomics of Micrurus alleni and Micrurus mosquitensis from the Caribbean region of Costa Rica reveals two divergent compositional patterns in New World elapids Fernández Ulate, Julián; Vargas Vargas, Nancy; Pla Ferrer, Davinia; Sasa Marín, Mahmood; Rey Suárez, Paola; Sanz, Libia; Gutiérrez, José María; Calvete Chornet, Juan José; Lomonte, Bruno (2015-12-01)Protein composition, toxicity, and neutralization of the venoms of Micrurus alleni and Micrurus mosquitensis, two sympatric monadal coral snakes found in humid environments of the Caribbean region of Costa Rica, were ...