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dc.creatorDíaz Oreiro, Cecilia
dc.creatorRivera Hidalgo, Jennifer
dc.creatorLomonte, Bruno
dc.creatorBonilla Murillo, Fabián
dc.creatorDiego García, Elia
dc.creatorCamacho Umaña, Erika
dc.creatorTytgat, Jan
dc.creatorSasa Marín, Mahmood
dc.date.accessioned2020-01-30T13:34:18Z
dc.date.available2020-01-30T13:34:18Z
dc.date.issued2019
dc.identifier.citationhttps://www.sciencedirect.com/science/article/pii/S0041010119304714?via%3Dihubes_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/80393
dc.description.abstractIn this study, we characterize the venom of Centruroides edwardsii, one of the most abundant scorpions in urban and rural areas of Costa Rica, in terms of its biochemical constituents and their biological activities. C. edwardsii venom is rich in peptides but also contains some higher molecular weight protein components. No phospholipase A2, hemolytic or fibrinogenolytic activities were found, but the presence of proteolytic and hyaluronidase enzymes was evidenced by zymography. Venom proteomic analysis indicates the presence of a hyaluronidase, several cysteine-rich secretory proteins, metalloproteinases and a peptidylglycine α-hydroxylating monooxygenase like-enzyme. It also includes peptides similar to the Kþ-channel blocker margatoxin, a dominant toxin in the venom of the related scorpion C. margaritatus. MS and N-terminal sequencing analysis also reveals the presence of Naþ-channel-modulating peptides with sequence similarity to orthologs present in other scorpion species of the genera Centruroides and Tityus. We purified the hyaluronidase (which co-eluted with an allergen 5-like CRiSP) and sequenced ~60% of this enzyme. We also sequenced some venom gland transcripts that include other cysteine-containing peptides and a Non-Disulfide Bridged Peptide (NDBP). Our in vivo experiments characterizing the effects on potential predators and prey show that C. edwardsii venom induces paralysis in several species of arthropods and geckos; crickets being the most sensitive and cockroaches and scorpions the most resistant organisms tested. Envenomation signs were also observed in mice, but no lethality was reached by intraperitoneal administration of this venom up to 120 μg/g body weight.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A5-048]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon, vol.171, pp.7-19es_ES
dc.subjectScorpion venomes_ES
dc.subjectPeptideses_ES
dc.subjectHyaluronidasees_ES
dc.subjectParalysises_ES
dc.subjectToxinses_ES
dc.titleVenom characterization of the bark scorpion Centruroides edwardsii (Gervais 1843): composition, biochemical activities and in vivo toxicity for potential preyes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doi10.1016/j.toxicon.2019.09.021
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Medicina::Escuela de Medicinaes_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biologíaes_ES
dc.identifier.codproyecto741-A5-048


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