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dc.creatorBarquero Quirós, Mirian
dc.creatorCunha Silva, Hugo
dc.creatorArcos Martínez, María Julia
dc.date.accessioned2020-03-06T20:46:49Z
dc.date.available2020-03-06T20:46:49Z
dc.date.issued2018
dc.identifier.citationhttps://www.tsijournals.com/abstract/superoxide-dismutase-based-biosensor-for-the-electrochemical-determination-of-epinephrine-13841.htmles_ES
dc.identifier.issn2277-288X
dc.identifier.urihttp://hdl.handle.net/10669/80689
dc.description.abstractNew amperometric biosensor for epinephrine was developed, using a screen printed carbon electrode with tetrathiafulvalene (5% v/w) incorporated into carbon ink (SPCTTFE). Electrodic surface was modified with nanoparticles of Au, Pt, Pd and Rh deposited on SPCTTFE by cyclic voltammetry. When using Pd nanoparticles, slope of EPI calibration curves was higher than the performed with the others NPs. The electrodeposited nanoparticles were evaluated with atomic force microscopy and electrochemical impedance spectroscopy was used to characterize electrode process. The developed superoxide dismutase-based biosensor was characterized by: limit of detection of 5.3 × 10-6 M (n=4), limit of quantification of 17.5 × 10-6 M (n=4), reproducibility with RSD of 2.8% (n=5), repeatability with RSD of 0.97% (n=3), accuracy was 102.8% with RSD of 4.3% (n=5). Linearity was obtained from 17.0 × 10-5 M to 8.59 × 10-4M. Interference study performed adding ascorbic acid and uric acid exhibits that the peak potential of both species are higher than the chosen for epinephrine analysis. Therefore developed biosensor and described in this paper, has been successfully applied to the determination of epinephrine in human gamma globulin and pharmaceutical samples. Developed biosensor offers easy assembly, excellent linearity and good performance parameters.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[804-B5-117]/UCR/Costa Ricaes_ES
dc.description.sponsorshipMinisterio de Ciencia e Innovación/[]/MICINN/Españaes_ES
dc.description.sponsorshipFondo Europeo de Desarrollo Regional/[TEC-TEC20013-40561-P]/FEDER/Españaes_ES
dc.description.sponsorshipFondo Europeo de Desarrollo Regional/[MUSSEL RTC-2015-4077-2]/FEDER/Españaes_ES
dc.language.isoen_USes_ES
dc.sourceActa Chimica and Pharmaceutica Indica, vol.8(3), pp.1-19es_ES
dc.subjectEpinephrinees_ES
dc.subjectSuperoxide dismutase enzymees_ES
dc.subjectScreen-printed electrodeses_ES
dc.subjectNanoparticleses_ES
dc.subjectElectrochemical impedance spectroscopyes_ES
dc.subjectAtomic force microscopyes_ES
dc.subjectBiosensores_ES
dc.titleSuperoxide dismutase based biosensor for the electrochemical determination of epinephrinees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Electroquímica y Energía Química (CELEQ)es_ES
dc.identifier.codproyecto804-B5-117


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