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dc.creatorBenavides Acevedo, Miguel Francisco
dc.creatorBolaños Villegas, Pablo Alberto
dc.date.accessioned2021-03-18T16:29:25Z
dc.date.available2021-03-18T16:29:25Z
dc.date.issued2021-03-12
dc.identifier.citationhttps://www.biorxiv.org/content/10.1101/2021.03.11.434975v3.fulles_ES
dc.identifier.urihttps://hdl.handle.net/10669/83094
dc.descriptionProyecto interdisciplinarioes_ES
dc.description.abstractPapaya (Carica papaya Linn.) is a tropical plant whose draft genome has been sequenced. Papaya produces large fruits rich in vitamins A and C and is an important cash crop in developing countries. Nonetheless, little is known about how the female gametophyte develops, how it is fertilized and how it develops into a mature seed containing an embryo and an endosperm. The Papaya female gametophyte displays a Polygonum-type architecture consisting of two synergid cells, an egg cell, a central cell, and three antipodal cells. Reports are available of the presumed existence of varieties in which cross fertilization is bypassed and autonomous development of embryos occurs (e.g., apomixis). In this study, we analyzed the development of female gametophytes in a commercial Hawaiian parental line and in the presumed apomictic Costa Rican line L1. Samples were collected before and after anthesis to compare the overall structure, size and transcriptional patterns of several genes that may be involved in egg and endosperm cell fate and proliferation. These genes were the putative papaya homologs of ARGONAUTE9 (AGO9), MEDEA (MEA), RETINOBLASTOMA RELATED-1 (RBR1), and SLOW WALKER-1 (SWA1). Our results suggest that its feasible to identify the contour of structural features of Polygonum-type development, and that in bagged female flowers of line L1 we might have observed autonomous development of embryo-like structures. Possible downregulation of papaya homologs for AGO9, MEA, RBR1 and SWA1 was observed in embryo sacs from line L1 before and after anthesis, which may suggest a tentative link between suspected apomixis and transcriptional downregulation of genes for RNA-directed DNA methylation, histone remodelers, and rRNA processing. Most notably, the large size of the papaya embryo sac suggests that it could be a cytological alternative to Arabidopsis thaliana for study. Significant variation in embryo sac size was observed between the varieties under study, suggesting wide differences in the genetic regulation of anatomical features.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[736-B5-A13]/UCR/Costa Ricaes_ES
dc.language.isoenges_ES
dc.sourcebioRviv, 2021.03.11.434975es_ES
dc.subjectCarica papayaes_ES
dc.subjectsexual reproductiones_ES
dc.subjectembryo saces_ES
dc.subjectembryo developmentes_ES
dc.subjectnatural variationes_ES
dc.subjectfood securityes_ES
dc.titleEmbryo development in Carica papaya Linnes_ES
dc.typepreprintes_ES
dc.identifier.doi10.1101/2021.03.11.434975
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Estación Experimental Agrícola Fabio Baudrit Moreno (EEAFBM)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biologíaes_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Jardín Botánico Lankester (JBL)es_ES
dc.identifier.codproyecto736-B5-A13


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