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dc.creatorLópez Dávila, Alfredo Jesús
dc.creatorWeber, Natalie
dc.creatorKraft, Theresia
dc.creatorMatinmehr, Faramarz
dc.creatorArias Hidalgo, Mariela Eugenia
dc.creatorFernández Ulate, Julián
dc.creatorLomonte, Bruno
dc.creatorGutiérrez, José María
dc.date.accessioned2022-02-25T15:42:00Z
dc.date.available2022-02-25T15:42:00Z
dc.date.issued2021-09
dc.identifier.citationhttps://www.nature.com/articles/s41598-021-98594-5es_ES
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/10669/85859
dc.description.abstractViperid snake venoms contain a unique family of cytotoxic proteins, the Lys49 PLA2 homologs, which are devoid of enzymatic activity but disrupt the integrity of cell membranes. They are known to induce skeletal muscle damage and are therefore named ‘myotoxins’. Single intact and skinned (devoid of membranes and cytoplasm but with intact sarcomeric proteins) rat cardiomyocytes were used to analyze the cytotoxic action of a myotoxin, from the venom of Bothrops asper. The toxin induced rapid hypercontraction of intact cardiomyocytes, associated with an increase in the cytosolic concentration of calcium and with cell membrane disruption. Hypercontraction of intact cardiomyocytes was abrogated by the myosin inhibitor para-aminoblebbistatin (AmBleb). No toxin-induced changes of key parameters of force development were observed in skinned cardiomyocytes. Thus, although myosin is a key effector of the observed hypercontraction, a direct effect of the toxin on the sarcomeric proteins -including the actomyosin complex- is not part of the mechanism of cytotoxicity. Owing to the sensitivity of intact cardiomyocytes to the cytotoxic action of myotoxin, this ex vivo model is a valuable tool to explore in further detail the mechanism of action of this group of snake venom toxins.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B5-602]/UCR/Costa Ricaes_ES
dc.language.isoenges_ES
dc.sourceScientific Reports, vol.11, 19452, pp.1-16.es_ES
dc.subjectMyotoxines_ES
dc.subjectCardiomyocytees_ES
dc.subjectCytotoxicityes_ES
dc.subjectSnake venomes_ES
dc.titleCytotoxicity of snake venom Lys49 PLA2-like myotoxin on rat cardiomyocytes ex vivo does not involve a direct action on the contractile apparatuses_ES
dc.typeartículo científicoes_ES
dc.identifier.doi10.1038/s41598-021-98594-5
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiologíaes_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Medicina::Escuela de Medicinaes_ES
dc.identifier.codproyecto741-B5-602


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