Show simple item record

dc.creatorValverde Barrantes, Juan Manuel
dc.creatorRodríguez Mora, Karina María
dc.creatorVillalta Arrieta, Mauren
dc.creatorHerrera Vega, María
dc.creatorSegura Ruiz, Álvaro
dc.creatorVargas Arroyo, Mariángela
dc.creatorMontero Villalobos, Mavis Lili
dc.creatorGutiérrez, José María
dc.creatorLeón Montero, Guillermo
dc.date.accessioned2022-03-01T14:20:04Z
dc.date.available2022-03-01T14:20:04Z
dc.date.issued2017
dc.identifier.citationhttps://www.sciencedirect.com/science/article/abs/pii/S0041010117300223?via%3Dihubes_ES
dc.identifier.issn0041-0101
dc.identifier.urihttps://hdl.handle.net/10669/85909
dc.description.abstractAdjuvant emulsions are widely used to enhance the antibody response of the animals used as immunoglobulin source for producing antivenoms. Usually, the adjuvant activity of emulsions is attributed both to their ability to trigger "danger" signals from cells in which they induce death, and to form depots from which immunogens are slowly released. However, there is contradictory evidence suggesting that adjuvant activity of emulsions is independent of the dispersion type and the rate of immunogen release. In order to test how physical properties of emulsions, composed of mineral oil and water, affect their ability to enhance the antibody response towards snake venoms, we compared water-in-oil (W/O) emulsions prepared at volume ratios of 70/30, 50/50 or 30/70, a 50/50 oil-in-water (O/W) emulsion, and a water-in-oil-in-water (W/O/W) multiple emulsion. Comparison included their droplet-size, viscosity, rate of immunogen release and ability to enhance the antibody response of mice immunized with the venom of the African viperid snake Echis ocellatus. It was found that all emulsions released a low amount of venom, and that the 50/50 (W/O) and the multiple emulsion (W/O/W) were those that induced the higher anti-venom antibody response. Our results suggest that the ability of emulsions to enhance the anti-venom response is not associated to their ability to form depots from which the venom is slowly released.es_ES
dc.description.sponsorshipInternational Foundation for Science/[B/5043-1]/IFS/Estados Unidoses_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B2-501]/UCR/Costa Ricaes_ES
dc.language.isoenges_ES
dc.sourceToxicon, vol.127, pp.106-111.es_ES
dc.subjectAdjuvantses_ES
dc.subjectEmulsionses_ES
dc.subjectAntibody responsees_ES
dc.subjectSnake venomes_ES
dc.subjectEchis ocellatuses_ES
dc.subjectVeneno de serpientees_ES
dc.subjectVENENOS ANIMALESes_ES
dc.titleComparison of the adjuvant activity of emulsions with different physicochemical properties on the antibody response towards the venom of West African carpet viper (Echis ocellatus)es_ES
dc.typeartículo científicoes_ES
dc.identifier.doi10.1016/j.toxicon.2017.01.011
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Electroquímica y Energía Química (CELEQ)es_ES
dc.identifier.codproyecto741-B2-501


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record