Hybrid mathematical modeling decodes the complexity of sphingolipid pathway to predict chemosensitivity
comunicación de congreso
Molina Mora, José Arturo
Quirós Barrantes, Steve
Kop Montero, Mariana
Mora Rodríguez, Rodrigo Antonio
Crespo Mariño, Juan Luis
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Sphingolipid (SL) signaling pathway is a complex biological system able to integrate different types of cellular stress signals related to induction of cell death pathways with special interest in cancer. This makes of the SL pathway a promising sensor of chemosensitivity and a target hub to overcome resistance. However, it is unclear how chemotherapeutic drugs can disturb the SL pathway and how the SL content modulates cellular fate. A hybrid mathematical model was proposed in order to integrate i) the metabolism of SL analogue (SM-BODIPY) modeled by an ordinary differential equation (ODE) approach, ii) a Gaussian mixture model (GMM) of the fluorescence features to identify how the SL pathway senses the effect of chemotherapeutic drugs and iii) a fuzzy logic model (FLM) to associate SL composition with cell viability by semi-quantitative rules. Altogether, this hybrid model approach was able to predict the cell viability of double experimental perturbations with chemotherapy, indicating that the SL pathway is a promising sensor to design strategies to overcome drug resistance in cancer.
External link to the item10.1109/IWOBI.2017.7985532
Forma parte de los trabajos presentado en el International Conference and Workshop on Bioinspired Intelligence (IWOBI). IEEE, Estados Unidos. Julio de 2017.
- Microbiología