A novel seed baiting technique for the epiphytic orchid Rhynchostele cervantesii, a means to acquire mycorrhizal fungi from protocorms
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Cruz Higareda, Jesús Bernardo
Luna Rosales, Bárbara Susana
Barba Álvarez, Amadeo
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Universidad de Costa Rica
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We developed a new and novel seed baiting technique sowing mature seeds of the epiphyitic orchid Rhynchostele cervantesii under natural conditions, We introduced a sponge in each package that may serve as a reservoir for water retention to benefit germination; In three of 22 packets we found protocorms in an early stage of development, six of wich were of sufficient size to warrant fungal isolations; Nine strains were isolated in pure culture and were inoculated on seeds and protocorms under in vitro conditions.
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