Clase 1 integrase ang genetic cassettes blaoxa and blatem among multidrug resistant Shigella isolates in Costa Rica

Abstract

Shigella is an important enteropathogen and the major cause of dysentery and diarrheal disease around the world. There are no studies related to the molecular detection of integrons among Shigella spp. isolates in Costa Rica. Here we are reporting for the first time the presence of class 1 integrase (intI-1) and extended spectrum β-lactamases genetic cassettes, blaOXA, and blaTEM among multidrug resistant Shigella isolates in Costa Rica. The antibiotic resistance pattern and presence of integrase class 1(intI-1) and genetic cassettes blaOXA and blaTEM were analyzed in 30 Shigella isolates (S. flexneri (83%) and S. sonnei (17%). Bacteria were collected at hospitals and cliniques in Costa Rica and exclusively from stools of individuals with diarrhea. from Costa Rica. Twenty nine out of 30 isolates were resistant to at least, one antibiotic. Multidrug resistance was observed in 26 out of 30 isolates (87%), and found to carry integrase class 1 (93%), and genetic cassettes blaOXA (76%) and blaTEM (7%).