Crystallization of the Lys49 PLA2 homologue, myotoxin II, from the venom of Atropoides nummifer

Watanabe, Leandra; Gava, Lisandra M.; Angulo Ugalde, Yamileth; Lomonte, Bruno; Arni, Raghuvir K.

doi:https://doi.org/10.1016/j.bbapap.2004.09.006

Crystallization of the Lys49 PLA2 homologue, myotoxin II, from the venom of Atropoides nummifer

Archivos

2004_PLA2_BBA_Watanabe_nummifer_crystallization.pdf (106,99 KB)

Fecha

2004-12

Authors

Watanabe, Leandra

Gava, Lisandra M.

Angulo Ugalde, Yamileth

Lomonte, Bruno

Arni, Raghuvir K.

DOI

https://doi.org/10.1016/j.bbapap.2004.09.006

Resumen

Myotoxin II, a Lys49 catalytically inactive phospholipase A2 homologue from Atropoides nummifer venom, was purified, characterized and crystallized. The crystals belongs to the tetragonal system, space group P43212, with unit cell parameters (a=b=68.66 and c=63.87 2). Diffraction data were collected to a resolution of 2.32 2. The crystal structure is currently being determined using molecular replacement techniques.